Antibody Name:	Anti-Somatostatin Antibody
Antibody SKU:	CAB9274
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human SST (NP_001039.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SW620, MCF7, Mouse brain, Mouse intestine, Rat stomach

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human SST (NP_001039.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KYFL AELL SEPN QTEN DALE PEDL SQAA EQDE MRLE LQRS ANSN PAMA PRER KAGC KNFF WKTF TSC
Gene ID:	6750
Uniprot:	P61278
Cellular Location:	Secreted
Calculated MW:	12kDa
Observed MW:	17kDa

Synonyms:

SST, SMST

Background:

The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells.

UniProt Protein Function:	SST: Somatostatin inhibits the release of somatotropin. Belongs to the somatostatin family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted; Hormone Chromosomal Location of Human Ortholog: 3q28 Cellular Component: extracellular space; cell soma; extracellular region Molecular Function:hormone activity Biological Process: response to drug; induction of apoptosis by hormones; response to amino acid stimulus; regulation of cell migration; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; synaptic transmission; hyperosmotic response; cell surface receptor linked signal transduction; cell-cell signaling; response to heat; response to steroid hormone stimulus; digestion; response to nutrient
NCBI Summary:	The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008]
UniProt Code:	P61278
NCBI GenInfo Identifier:	4507243
NCBI Gene ID:	6750
NCBI Accession:	NP_001039.1
UniProt Related Accession:	P61278
Molecular Weight:
NCBI Full Name:	somatostatin preproprotein
NCBI Synonym Full Names:	somatostatin
NCBI Official Symbol:	SST
NCBI Official Synonym Symbols:	SMST
NCBI Protein Information:	somatostatin
UniProt Protein Name:	Somatostatin
UniProt Synonym Protein Names:	Growth hormone release-inhibiting factorCleaved into the following 2 chains:Somatostatin-28; Somatostatin-14
Protein Family:	Sucrose synthase
UniProt Gene Name:	SST
UniProt Entry Name:	SMS_HUMAN

	Western blot analysis of extracts of various cell lines, using SST antibody (CAB9274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human appendix using SST antibody (CAB9274) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using SST antibody (CAB9274) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of rat pancreas using SST Rabbit pAb (CAB9274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug SST antibody (CAB9274). Western blot was performed from the immunoprecipitate using SST antibody (CAB9274) at a dilition of 1:1000.