Developmental Biology
Anti-SOCS1 Antibody (CAB7754)
- SKU:
- CAB7754
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-SOCS1 Antibody |
| Antibody SKU: | CAB7754 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human SOCS1 (NP_003736.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human SOCS1 (NP_003736.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVAH NQVA ADNA VSTA AEPR RRPE PSSS SSSS PAAP ARPR PCPA VPAP APGD THFR TFRS HADY RRIT RASA LLDA CGFY WGPL SVHG AHER LRAE PVGT FLVR DSRQ RNCF FALS VKMA SGPT SIRV HFQA GRFH LDGS RESF DCLF ELLE HYVA APRR MLGA PLRQ RRVR PLQE LCRQ RIVA TVGR ENLA RIPL NPVL RDYL SSFP FQI |
| Gene ID: | 8651 |
| Uniprot: | O15524 |
| Cellular Location: | Cytoplasmic vesicle, Nucleus |
| Calculated MW: | 23kDa |
| Observed MW: | 24kDa |
| Synonyms: | SOCS1, CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3, TIP-3 |
| Background: | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. |
| UniProt Protein Function: | SOCS1: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR. By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families. Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 16p13.13 Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; nucleus Molecular Function:insulin-like growth factor receptor binding; protein binding; protein kinase inhibitor activity; kinase inhibitor activity; protein kinase binding Biological Process: fat cell differentiation; regulation of protein amino acid phosphorylation; cytokine and chemokine mediated signaling pathway; regulation of growth; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of insulin receptor signaling pathway; protein ubiquitination; negative regulation of protein kinase activity; JAK-STAT cascade; negative regulation of JAK-STAT cascade |
| NCBI Summary: | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15524 |
| NCBI GenInfo Identifier: | 20178097 |
| NCBI Gene ID: | 8651 |
| NCBI Accession: | O15524.1 |
| UniProt Secondary Accession: | O15524,O15097, Q9NSA7, |
| UniProt Related Accession: | O15524 |
| Molecular Weight: | |
| NCBI Full Name: | Suppressor of cytokine signaling 1 |
| NCBI Synonym Full Names: | suppressor of cytokine signaling 1 |
| NCBI Official Symbol: | SOCS1 |
| NCBI Official Synonym Symbols: | JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1 |
| NCBI Protein Information: | suppressor of cytokine signaling 1; TIP-3; JAK binding protein; JAK-binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; cytokine-inducible SH2 protein 1 |
| UniProt Protein Name: | Suppressor of cytokine signaling 1 |
| UniProt Synonym Protein Names: | JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3 |
| Protein Family: | Suppressor of cytokine signaling |
| UniProt Gene Name: | SOCS1 |
| UniProt Entry Name: | SOCS1_HUMAN |
 | Western blot analysis of extracts of Raji cells, using SOCS1 antibody (CAB7754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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