Metabolism Antibodies 2
Anti-SOAT1 Antibody (CAB6311)
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- SKU:
- CAB6311
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-SOAT1 Antibody |
| Antibody SKU: | CAB6311 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | H460, HeLa, SW480, Mouse stomach, Mouse liver, Rat liver, Rat stomach |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVGE EKMS LRNR LSKS RENP EEDE DQRN PAKE SLET PSNG RIDI KQLI AKKI KLTA EAEE LKPF FMKE VGSH FDDF VTNL IEKS ASLD NGGC ALTT FSVL EGEK NNHR AKDL RAPP EQGK IFIA RRSL LD |
| Gene ID: | 6646 |
| Uniprot: | P35610 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 57kDa/58kDa/64kDa |
| Observed MW: | 55kDa |
| Synonyms: | SOAT1, ACACT, ACAT, ACAT-1, ACAT1, SOAT, STAT |
| Background: | The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | SOAT1: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; EC 2.3.1.26; Transferase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 1q25 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; acyl-CoA binding; sterol O-acyltransferase activity; cholesterol binding Biological Process: cholesterol metabolic process; cholesterol homeostasis; cholesterol efflux; positive regulation of amyloid precursor protein biosynthetic process |
| NCBI Summary: | The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | P35610 |
| NCBI GenInfo Identifier: | 33302623 |
| NCBI Gene ID: | 6646 |
| NCBI Accession: | P35610.3 |
| UniProt Secondary Accession: | P35610,Q5T0X4, Q8N1E4, A6NC40, A8K3P4, A9Z1V7, B4DU95 |
| UniProt Related Accession: | P35610 |
| Molecular Weight: | 550 |
| NCBI Full Name: | Sterol O-acyltransferase 1 |
| NCBI Synonym Full Names: | sterol O-acyltransferase 1 |
| NCBI Official Symbol: | SOAT1 |
| NCBI Official Synonym Symbols: | ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1 |
| NCBI Protein Information: | sterol O-acyltransferase 1; acyl-coenzyme A:cholesterol acyltransferase 1; sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1 |
| UniProt Protein Name: | Sterol O-acyltransferase 1 |
| UniProt Synonym Protein Names: | Acyl-coenzyme A:cholesterol acyltransferase 1; ACAT-1; Cholesterol acyltransferase 1 |
| Protein Family: | Sterol O-acyltransferase |
| UniProt Gene Name: | SOAT1 |
| UniProt Entry Name: | SOAT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SOAT1 antibody (CAB6311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using SOAT1 Rabbit pAb (CAB6311) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using SOAT1 Rabbit pAb (CAB6311) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using SOAT1 Rabbit pAb (CAB6311) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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