Antibody Name:	Anti-SNX13 Antibody
Antibody SKU:	CAB12821
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-230 of human SNX13 (NP_055947.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-230 of human SNX13 (NP_055947.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGKT NSEK YLEQ CEHS FLPP TSPG VPKC LEEM KREA RTIK IDRR LTGA NIID EPLQ QVIQ FSLR DYVQ YWYY TLSD DESF LLEI RQTL QNAL IQFA TRSK EIDW QPYF TTRI VDDF GTHL RVFR KAQQ KITE KDDQ VKGT AEDL VDTF FEVE VEME KEVC RDLV CTSP KDEE GFLR DLCE V
Gene ID:	23161
Uniprot:	Q9Y5W8
Cellular Location:	Cytoplasmic side, Early endosome membrane, Peripheral membrane protein
Calculated MW:	110kDa/112kDa
Observed MW:	112kDa

Synonyms:

SNX13, RGS-PX1

Background:

This gene encodes a PHOX domain- and RGS domain-containing protein that belongs to the sorting nexin (SNX) family and the regulator of G protein signaling (RGS) family. The PHOX domain is a phosphoinositide binding domain, and the SNX family members are involved in intracellular trafficking. The RGS family members are regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. The RGS domain of this protein interacts with G alpha(s), accelerates its GTP hydrolysis, and attenuates G alpha(s)-mediated signaling. Overexpression of this protein delayes lysosomal degradation of the epidermal growth factor receptor. Because of its bifunctional role, this protein may link heterotrimeric G protein signaling and vesicular trafficking.

UniProt Protein Function:	SNX13: May be involved in several stages of intracellular trafficking. May play a role in endosome homeostasis. Acts as a GAP for Galphas. Belongs to the sorting nexin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 7p21.1 Cellular Component: early endosome Molecular Function:phosphatidylinositol 3-phosphate binding; phosphoinositide binding Biological Process: intracellular protein transport; positive regulation of GTPase activity
NCBI Summary:	This gene encodes a PHOX domain- and RGS domain-containing protein that belongs to the sorting nexin (SNX) family and the regulator of G protein signaling (RGS) family. The PHOX domain is a phosphoinositide binding domain, and the SNX family members are involved in intracellular trafficking. The RGS family members are regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. The RGS domain of this protein interacts with G alpha(s), accelerates its GTP hydrolysis, and attenuates G alpha(s)-mediated signaling. Overexpression of this protein delayes lysosomal degradation of the epidermal growth factor receptor. Because of its bifunctional role, this protein may link heterotrimeric G protein signaling and vesicular trafficking. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y5W8
NCBI GenInfo Identifier:	24418867
NCBI Gene ID:	23161
NCBI Accession:	Q9Y5W8.4
UniProt Secondary Accession:	Q9Y5W8,O94821, Q8WVZ2, Q8WXH8, B2RCI9,
UniProt Related Accession:	Q9Y5W8
Molecular Weight:	112kDa
NCBI Full Name:	Sorting nexin-13
NCBI Synonym Full Names:	sorting nexin 13
NCBI Official Symbol:	SNX13
NCBI Official Synonym Symbols:	RGS-PX1
NCBI Protein Information:	sorting nexin-13
UniProt Protein Name:	Sorting nexin-13
UniProt Synonym Protein Names:	RGS domain- and PHOX domain-containing protein; RGS-PX1
Protein Family:	Sorting nexin
UniProt Gene Name:	SNX13

	Western blot analysis of extracts of various cell lines, using SNX13 antibody (CAB12821) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of mouse brain using SNX13 antibody (CAB12821) at dilution of 1:100. Blue: DAPI for nuclear staining.