Antibody Name:	Anti-SNW1 Antibody
Antibody SKU:	CAB14580
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-536 of human SNW1 (NP_036377.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, 293T, HeLa, HepG2, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-536 of human SNW1 (NP_036377.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EAVE MRAQ VERK MAQK EKEK HEEK LREM AQKA RERR AGIK THVE KEDG EARE RDEI RHDR RKER QHDR NLSR AAPD KRSK LQRN ENRD ISEV IALG VPNP RTSN EVQY DQRL FNQS KGMD SGFA GGED EIYN VYDQ AWRG GKDM AQSI YRPS KNLD KDMY GDDL EARI KTNR FVPD KEFS GSDR RQRG REGP VQFE EDPF GLDK FLEE AKQH GGSK RPSD SSRP KEHE HEGK KRRK E
Gene ID:	22938
Uniprot:	Q13573
Cellular Location:	Nucleus
Calculated MW:	61kDa
Observed MW:	70kDa/75kDa

Synonyms:

SNW1, Bx42, NCOA-62, PRPF45, Prp45, SKIIP, SKIP, FUN20, SKIP1

Background:

This gene, a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also function as a splicing factor by interacting with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Skip: Involved in vitamin D-mediated transcription. Can function as a splicing factor in pre-mRNA splicing. Belongs to the SNW family.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Spliceosome Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: nuclear matrix; nucleoplasm; nucleus; spliceosome; transcription elongation factor complex b Molecular Function:Notch binding; nuclear hormone receptor binding; protein binding; retinoic acid receptor binding; SMAD binding; transcription coactivator activity; transcription corepressor activity; vitamin D receptor binding Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; Notch signaling pathway; nuclear mRNA splicing, via spliceosome; positive regulation of histone H3-K4 methylation; positive regulation of neurogenesis; positive regulation of nuclear mRNA splicing, via spliceosome; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of retinoic acid receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter
NCBI Summary:	This gene, a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also function as a splicing factor by interacting with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
UniProt Code:	Q13573
NCBI GenInfo Identifier:	2500813
NCBI Gene ID:	22938
NCBI Accession:	Q13573.1
UniProt Secondary Accession:	Q13573,Q13483, Q32N03, Q5D0D6, A8K8A9,
UniProt Related Accession:	Q13573
Molecular Weight:	61,494 Da
NCBI Full Name:	SNW domain-containing protein 1
NCBI Synonym Full Names:	SNW domain containing 1
NCBI Official Symbol:	SNW1
NCBI Official Synonym Symbols:	Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62
NCBI Protein Information:	SNW domain-containing protein 1
UniProt Protein Name:	SNW domain-containing protein 1
UniProt Synonym Protein Names:	Nuclear protein SkiP; Nuclear receptor coactivator NCoA-62; Ski-interacting protein
Protein Family:	SNW domain-containing protein
UniProt Gene Name:	SNW1
UniProt Entry Name:	SNW1_HUMAN

	Western blot analysis of extracts of various cell lines, using SNW1 antibody (CAB14580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using SNW1 antibody (CAB14580) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using SNW1 antibody (CAB14580) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using SNW1 antibody (CAB14580) at dilution of 1:100. Blue: DAPI for nuclear staining.