Epigenetics & Nuclear Signaling Antibodies 1
Anti-SNRPF Antibody (CAB12162)
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- SKU:
- CAB12162
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-SNRPF Antibody |
| Antibody SKU: | CAB12162 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human SNRPF (NP_003086.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HT-29, 293T, LO2, SGC-7901, Mouse brain, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human SNRPF (NP_003086.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSLP LNPK PFLN GLTG KPVM VKLK WGME YKGY LVSV DGYM NMQL ANTE EYID GALS GHLG EVLI RCNN VLYI RGVE EEEE DGEM RE |
| Gene ID: | 6636 |
| Uniprot: | P62306 |
| Cellular Location: | Cytoplasm, Nucleus, cytosol |
| Calculated MW: | 9kDa |
| Observed MW: | 12kDa |
| Synonyms: | SNRPF, SMF, Sm-F, snRNP-F |
| Background: |
| UniProt Protein Function: | snRNP F: Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; RNA splicing; Spliceosome Chromosomal Location of Human Ortholog: 12q23.1 Cellular Component: cytosol; nucleoplasm; small nuclear ribonucleoprotein complex; snRNP U1; spliceosome; U12-dependent spliceosome Molecular Function:protein binding; RNA binding Biological Process: histone mRNA metabolic process; nuclear import; nuclear mRNA splicing, via spliceosome; RNA splicing; spliceosomal snRNP biogenesis; termination of RNA polymerase II transcription |
| UniProt Code: | P62306 |
| NCBI GenInfo Identifier: | 61237391 |
| NCBI Gene ID: | 6636 |
| NCBI Accession: | P62306.1 |
| UniProt Secondary Accession: | P62306,Q15356, Q6IBQ1, Q6P4I0, A2VCR2, B2R498, |
| UniProt Related Accession: | P62306 |
| Molecular Weight: | 10kDa |
| NCBI Full Name: | Small nuclear ribonucleoprotein F |
| NCBI Synonym Full Names: | small nuclear ribonucleoprotein polypeptide F |
| NCBI Official Symbol: | SNRPF |
| NCBI Official Synonym Symbols: | SMF; Sm-F; snRNP-F |
| NCBI Protein Information: | small nuclear ribonucleoprotein F |
| UniProt Protein Name: | Small nuclear ribonucleoprotein F |
| UniProt Synonym Protein Names: | Sm protein F; Sm-F; SmF |
| UniProt Gene Name: | SNRPF |
 | Western blot analysis of extracts of various cell lines, using SNRPF antibody (CAB12162) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using SNRPF Rabbit pAb (CAB12162) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using SNRPF Rabbit pAb (CAB12162) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using SNRPF Rabbit pAb (CAB12162) at dilution of 1:200 (40x lens). |
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