Antibody Name:	Anti-SNRPB Antibody
Antibody SKU:	CAB2009
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SNRPB (NP_003082.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HT-29, Jurkat, HepG2, Mouse brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SNRPB (NP_003082.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTVG KSSK MLQH IDYR MRCI LQDG RIFI GTFK AFDK HMNL ILCD CDEF RKIK PKNS KQAE REEK RVLG LVLL RGEN LVSM TVEG PPPK DTGI ARVP LAGA
Gene ID:	6628
Uniprot:	P14678
Cellular Location:	Cytoplasm, Nucleus, cytosol
Calculated MW:	23kDa/24kDa/30kDa
Observed MW:	30kDa

Synonyms:

SNRPB, CCMS, COD, SNRPB1, Sm-B/B, SmB/SmB, snRNP-B

Background:

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene.

UniProt Protein Function:	Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. As part of the U7 snRNP it is involved in histone 3'-end processing.
NCBI Summary:	The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P14678
NCBI GenInfo Identifier:	134037
NCBI Gene ID:	6628
NCBI Accession:	P14678.2
UniProt Secondary Accession:	P14678,Q15490, Q6IB35, Q9UIS5,
UniProt Related Accession:	P14678
Molecular Weight:	30,032 Da
NCBI Full Name:	Small nuclear ribonucleoprotein-associated proteins B and B'
NCBI Synonym Full Names:	small nuclear ribonucleoprotein polypeptides B and B1
NCBI Official Symbol:	SNRPB
NCBI Official Synonym Symbols:	COD; CCMS; SNRPB1; SmB/B'; Sm-B/B'; snRNP-B; SmB/SmB'
NCBI Protein Information:	small nuclear ribonucleoprotein-associated proteins B and B'
UniProt Protein Name:	Small nuclear ribonucleoprotein-associated proteins B and B'
UniProt Synonym Protein Names:	Sm protein B/B'; Sm-B/B'; SmB/B'
Protein Family:	Small nuclear ribonucleoprotein-associated protein
UniProt Gene Name:	SNRPB
UniProt Entry Name:	RSMB_HUMAN

	Western blot analysis of extracts of various cell lines, using SNRPB antibody (CAB2009) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat spleen using SNRPB Rabbit pAb (CAB2009) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using SNRPB Rabbit pAb (CAB2009) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using SNRPB Rabbit pAb (CAB2009) at dilution of 1:100 (40x lens).