Description
| Antibody Name: | Anti-SNRPA Antibody |
| Antibody SKU: | CAB6410 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, 293T, A-431, MCF7, Mouse brain, Mouse testis, Mouse thymus, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human SNRPA (NP_004587.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAVP ETRP NHTI YINN LNEK IKKD ELKK SLYA IFSQ FGQI LDIL VSRS LKMR GQAF VIFK EVSS ATNA LRSM QGFP FYDK PMRI QYAK TDSD IIAK MKGT FVER DRKR EKRK PKSQ ETPA TKKA VQGG GATP VVGA VQGP VPGM PPMT QAPR IMHH MPGQ PPYM PPPG MIPP PGLA PGQI PPGA MPPQ QLMP GQMP PAQP LSEN PPNH ILFL TNLP EETN ELML SMLF NQFP GFKE VRLV PGRH DIAF VEFD NEVQ AGAA RDAL QGFK ITQN NAMK ISFA KK |
| Gene ID: | 6626 |
| Uniprot: | P09012 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa |
| Observed MW: | 31kDa |
| Synonyms: | SNRPA, Mud1, U1-A, U1A |
| Background: | The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. |
| UniProt Protein Function: | Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs. |
| NCBI Summary: | The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010] |
| UniProt Code: | P09012 |
| NCBI GenInfo Identifier: | 134092 |
| NCBI Gene ID: | 6626 |
| NCBI Accession: | P09012.3 |
| UniProt Related Accession: | P09012 |
| Molecular Weight: | 282 |
| NCBI Full Name: | U1 small nuclear ribonucleoprotein A |
| NCBI Synonym Full Names: | small nuclear ribonucleoprotein polypeptide A |
| NCBI Official Symbol: | SNRPA |
| NCBI Official Synonym Symbols: | U1A; Mud1; U1-A |
| NCBI Protein Information: | U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1 snRNP-specific protein A; U1 small nuclear RNP-specific A |
| UniProt Protein Name: | U1 small nuclear ribonucleoprotein A |
| Protein Family: | U1 small nuclear ribonucleoprotein |
| UniProt Gene Name: | SNRPA |
| UniProt Entry Name: | SNRPA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SNRPA antibody (CAB6410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody (CAB6410) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody (CAB6410) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody (CAB6410) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody (CAB6410) at dilution of 1:100 (40x lens). |
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