Epigenetics & Nuclear Signaling Antibodies 1

Anti-SNRNP35 Antibody (CAB13211)

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SKU:
CAB13211
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SNRNP35 Antibody
Antibody SKU:CAB13211
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNRNP35 (NP_073208.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNRNP35 (NP_073208.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNDW MPIA KEYD PLKA GSID GTDE DPHD RAVW RAML ARYV PNKG VIGD PLLT LFVA RLNL QTKE DKLK EVFS RYGD IRRL RLVR DLVT GFSK GYAF IEYK EERA VIKA YRDA DGLV IDQH EIFV DYEL ERTL KGWI PRRL GGGL GGKK ES
Gene ID:11066
Uniprot:Q16560
Cellular Location:Nucleus
Calculated MW:29kDa
Observed MW:35kDa
Synonyms:SNRNP35, HM-1, U1SNRNPBP
Background:The protein encoded by this gene is a homolog of the U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal half is rich in Arg/Asp and Arg/Glu dipeptides, which is a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. Alternative splicing results in multiple transcript variants encoding two distinct isoforms and representing a non-protein coding variant.
UniProt Code:Q16560
NCBI GenInfo Identifier:13027642
NCBI Gene ID:11066
NCBI Accession:NP_073208.1
UniProt Secondary Accession:Q16560,Q5XKN9, A8K262,
UniProt Related Accession:Q16560
Molecular Weight:29,450 Da
NCBI Full Name:U11/U12 small nuclear ribonucleoprotein 35 kDa protein isoform a
NCBI Synonym Full Names:small nuclear ribonucleoprotein 35kDa (U11/U12)
NCBI Official Symbol:SNRNP35  
NCBI Official Synonym Symbols:HM-1; U1SNRNPBP  
NCBI Protein Information:U11/U12 small nuclear ribonucleoprotein 35 kDa protein; U11/U12-35K; protein HM-1; U11/U12 snRNP 35K; U11/U12 snRNP 35 kDa protein; U1 snRNP binding protein homolog; U1 snRNP-binding protein homolog; U1-snRNP binding protein homolog
UniProt Protein Name:U11/U12 small nuclear ribonucleoprotein 35 kDa protein
UniProt Synonym Protein Names:Protein HM-1; U1 snRNP-binding protein homolog
Protein Family:U11/U12 small nuclear ribonucleoprotein 35 kDa protein
UniProt Gene Name:SNRNP35  
UniProt Entry Name:U1SBP_HUMAN
Anti-SNRNP35 Antibody (CAB13211)Western blot analysis of extracts of various cell lines, using SNRNP35 antibody (CAB13211) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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