Cell Biology Antibodies 2

Anti-SNF8 Antibody (CAB10583)

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SKU:
CAB10583

Description

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Antibody Name:Anti-SNF8 Antibody
Antibody SKU:CAB10583
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNF8 (NP_009172.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNF8 (NP_009172.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MHRR GVGA GAIA KKKL AEAK YKER GTVL AEDQ LAQM SKQL DMFK TNLE EFAS KHKQ EIRK NPEF RVQF QDMC ATIG VDPL ASGK GFWS EMLG VGDF YYEL GVQI IEVC LALK HRNG GLIT LEEL HQQV LKGR GKFA QDVS QDDL IRAI KKLK ALGT GFGI IPVG GTYL IQSV PAEL NMDH TVVL QLAE KNGY VTVS EIKA SLKW ETER ARQV LEHL LKEG LAWL DLQA PGEA HYWL PALF TDLY SQEI TAEE AREA LP
Gene ID:11267
Uniprot:Q96H20
Cellular Location:Cytoplasm, Endosome membrane, Late endosome membrane, Nucleus
Calculated MW:28kDa
Observed MW:30kDa
Synonyms:SNF8, Dot3, EAP30, VPS22
Background:The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SNF8: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Belongs to the SNF8 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytoplasm; cytosol; endosome membrane; late endosome membrane; membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome; transcription factor complex

Molecular Function:channel regulator activity; protein binding; protein C-terminus binding; protein homodimerization activity; protein N-terminus binding; transcription factor binding

Biological Process: early endosome to late endosome transport; endocytic recycling; endosome transport; macroautophagy; positive regulation of protein catabolic process; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; regulation of MAP kinase activity; regulation of protein catabolic process; regulation of transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
UniProt Code:Q96H20
NCBI GenInfo Identifier:73919323
NCBI Gene ID:11267
NCBI Accession:Q96H20.1
UniProt Secondary Accession:Q96H20,Q8IXY3, Q9UN50,
UniProt Related Accession:Q96H20
Molecular Weight:29kDa
NCBI Full Name:Vacuolar-sorting protein SNF8
NCBI Synonym Full Names:SNF8, ESCRT-II complex subunit
NCBI Official Symbol:SNF8  
NCBI Official Synonym Symbols:Dot3; EAP30; VPS22  
NCBI Protein Information:vacuolar-sorting protein SNF8
UniProt Protein Name:Vacuolar-sorting protein SNF8
UniProt Synonym Protein Names:ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22
Protein Family:Vacuolar-sorting protein
UniProt Gene Name:SNF8  
Anti-SNF8 Antibody (CAB10583)Western blot analysis of extracts of various cell lines, using SNF8 antibody (CAB10583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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