Name: Anti-SNAPIN Antibody (CAB19980)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19980
Availability: OutOfStock

Antibody Name:	Anti-SNAPIN Antibody [KO Validated]
Antibody SKU:	CAB19980
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human SNAPIN.

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant protein of human SNAPIN.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	23557
Uniprot:	O95295
Cellular Location:	Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Golgi apparatus membrane, Membrane, Peripheral membrane protein, perinuclear region, secretory vesicle, synaptic vesicle membrane
Calculated MW:	14kDa
Observed MW:	17KDa

Anti-SNAPIN Antibody [KO Validated] (CAB19980)

Western blot analysis of extracts from normal (control) and SNAPIN knockout (KO) HeLa cells, using SNAPIN antibody (CAB19980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

SNAPIN, BLOC1S7, BLOS7, BORCS3, SNAPAP

Background:

The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SNAPIN: a component of the SNARE complex of proteins that is required for synaptic vesicle docking and fusion. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. Phosphorylation by PKA modulates its interaction with the SNARE complex.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: perinuclear region of cytoplasm; secretory granule; synapse; synaptic vesicle Molecular Function:protein binding; SNARE binding Biological Process: anterograde axon cargo transport; anterograde synaptic vesicle transport; endosome to lysosome transport; lysosome localization; neurite development; neurotransmitter secretion; regulation of protein binding; synaptic vesicle exocytosis; synaptic vesicle transport
NCBI Summary:	The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
UniProt Code:	O95295
NCBI GenInfo Identifier:	41017846
NCBI Gene ID:	23557
NCBI Accession:	O95295.1
UniProt Secondary Accession:	O95295,Q5SXU8, D3DV56,
UniProt Related Accession:	O95295
Molecular Weight:	15kDa
NCBI Full Name:	SNARE-associated protein Snapin
NCBI Synonym Full Names:	SNAP associated protein
NCBI Official Symbol:	SNAPIN
NCBI Official Synonym Symbols:	BLOS7; BORCS3; SNAPAP; BLOC1S7
NCBI Protein Information:	SNARE-associated protein Snapin
UniProt Protein Name:	SNARE-associated protein Snapin
UniProt Synonym Protein Names:	Biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 subunit 7; Synaptosomal-associated protein 25-binding protein; SNAP-associated protein
Protein Family:	SNAPIN protein
UniProt Gene Name:	SNAPIN