Description
| Antibody Name: | Anti-Snail Antibody |
| Antibody SKU: | CAB5544 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Snail (NP_005976.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Snail (NP_005976.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RTHT GEKP FSCP HCSR AFAD RSNL RAHL QTHS DVKK YQCQ ACAR TFSR MSLL HKHQ ESGC SGCP R |
| Gene ID: | 6615 |
| Uniprot: | O95863 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 29kDa |
| Observed MW: | 29kDa |
| Synonyms: | SNAI1, SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1 |
| Background: | The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. |
| UniProt Protein Function: | Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 20q13.2 Cellular Component: cytoplasm; nucleus Molecular Function:kinase binding; protein binding Biological Process: epithelial to mesenchymal transition; mesoderm formation; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; positive regulation of cell migration; positive regulation of transcription, DNA-dependent |
| NCBI Summary: | The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95863 |
| NCBI GenInfo Identifier: | 12644089 |
| NCBI Gene ID: | 6615 |
| NCBI Accession: | O95863.2 |
| UniProt Secondary Accession: | O95863,Q9P113, Q9UBP7, Q9UHH7, B2R842, |
| UniProt Related Accession: | O95863 |
| Molecular Weight: | 29,083 Da |
| NCBI Full Name: | Zinc finger protein SNAI1 |
| NCBI Synonym Full Names: | snail family transcriptional repressor 1 |
| NCBI Official Symbol: | SNAI1Â Â |
| NCBI Official Synonym Symbols: | SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1Â Â |
| NCBI Protein Information: | zinc finger protein SNAI1 |
| UniProt Protein Name: | Zinc finger protein SNAI1 |
| UniProt Synonym Protein Names: | Protein snail homolog 1; Protein sna |
| Protein Family: | Protein snail |
| UniProt Gene Name: | SNAI1Â Â |
| UniProt Entry Name: | SNAI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Snail antibody (CAB5544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using Snail antibody (CAB5544) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using Snail antibody (CAB5544) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using Snail antibody (CAB5544) at dilution of 1:100 (40x lens). |
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