Cell Biology Antibodies 2

Anti-Snail Antibody (CAB12301)

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SKU:
CAB12301
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Snail Antibody
Antibody SKU:CAB12301
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Snail (NP_005976.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, Mouse lung, Mouse heart, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Snail (NP_005976.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RTHT GEKP FSCP HCSR AFAD RSNL RAHL QTHS DVKK YQCQ ACAR TFSR MSLL HKHQ ESGC SGCP R
Gene ID:6615
Uniprot:O95863
Cellular Location:Cytoplasm, Nucleus
Calculated MW:29kDa
Observed MW:29kDa
Synonyms:SNAI1, SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1
Background:The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
UniProt Protein Function:Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: cytoplasm; nucleus

Molecular Function:kinase binding; protein binding

Biological Process: epithelial to mesenchymal transition; mesoderm formation; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; positive regulation of cell migration; positive regulation of transcription, DNA-dependent

NCBI Summary:The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]
UniProt Code:O95863
NCBI GenInfo Identifier:12644089
NCBI Gene ID:6615
NCBI Accession:O95863.2
UniProt Secondary Accession:O95863,Q9P113, Q9UBP7, Q9UHH7, B2R842,
UniProt Related Accession:O95863
Molecular Weight:29,083 Da
NCBI Full Name:Zinc finger protein SNAI1
NCBI Synonym Full Names:snail family transcriptional repressor 1
NCBI Official Symbol:SNAI1  
NCBI Official Synonym Symbols:SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1  
NCBI Protein Information:zinc finger protein SNAI1
UniProt Protein Name:Zinc finger protein SNAI1
UniProt Synonym Protein Names:Protein snail homolog 1; Protein sna
Protein Family:Protein snail
UniProt Gene Name:SNAI1  
UniProt Entry Name:SNAI1_HUMAN
Anti-Snail Antibody (CAB12301)Western blot analysis of extracts of various cell lines, using Snail antibody (CAB12301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-Snail Antibody (CAB12301)Confocal immunofluorescence analysis of U2OS cells using Snail Polyclonal Antibody (CAB12301) at dilution of 1:100. Blue: DAPI for nuclear staining.
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