Name: Anti-SMYD3 Antibody (CAB7309)
Brand: Cell Biology Antibodies 11
SKU: CAB7309
Availability: OutOfStock

Antibody Name:	Anti-SMYD3 Antibody
Antibody SKU:	CAB7309
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-369 of human SMYD3 (NP_073580.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Mouse brain, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-369 of human SMYD3 (NP_073580.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PSIS LLNH SCDP NCSI VFNG PHLL LRAV RDIE VGEE LTIC YLDM LMTS EERR KQLR DQYC FECD CFRC QTQD KDAD MLTG DEQV WKEV QESL KKIE ELKA HWKW EQVL AMCQ AIIS SNSE RLPD INIY QLKV LDCA MDAC INLG LLEE ALFY GTRT MEPY RIFF PGSH PVRG VQVM KVGK LQLH QGMF PQAM KNLR LAFD IMRV THGR EHSL IEDL ILLL EECD ANIR AS
Gene ID:	64754
Uniprot:	Q9H7B4
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	29kDa/42kDa/49kDa
Observed MW:	49kDa

Synonyms:	SMYD3, KMT3E, ZMYND1, ZNFN3A1, bA74P14.1
Background:	This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SMYD3: Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase Chromosomal Location of Human Ortholog: 1q44 Cellular Component: nucleoplasm; cytoplasm Molecular Function:metal ion binding; histone-lysine N-methyltransferase activity Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; establishment of protein localization; myotube cell development; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation
NCBI Summary:	This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9H7B4
NCBI GenInfo Identifier:	212276523
NCBI Gene ID:	64754
NCBI Accession:	Q9H7B4.4
UniProt Secondary Accession:	Q9H7B4,Q86TL8, Q8N5Z6, Q96AI5, A8K0P0, B1AN38,
UniProt Related Accession:	Q9H7B4
Molecular Weight:	428
NCBI Full Name:	Histone-lysine N-methyltransferase SMYD3
NCBI Synonym Full Names:	SET and MYND domain containing 3
NCBI Official Symbol:	SMYD3
NCBI Official Synonym Symbols:	KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
NCBI Protein Information:	histone-lysine N-methyltransferase SMYD3; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; SET and MYND domain-containing protein 3; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing)
UniProt Protein Name:	Histone-lysine N-methyltransferase SMYD3
UniProt Synonym Protein Names:	SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1
Protein Family:	Histone-lysine N-methyltransferase
UniProt Gene Name:	SMYD3
UniProt Entry Name:	SMYD3_HUMAN

	Western blot analysis of extracts of various cell lines, using SMYD3 antibody (CAB7309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of A-549 cells using SMYD3 antibody (CAB7309).