Name: Anti-SMUG1 Antibody (CAB10166)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB10166
Availability: OutOfStock

Antibody Name:	Anti-SMUG1 Antibody
Antibody SKU:	CAB10166
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMUG1 (NP_055126.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:1000
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, Mouse kidney, Mouse liver, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMUG1 (NP_055126.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPQA FLLG SIHE PAGA LMEP QPCP GSLA ESFL EEEL RLNA ELSQ LQFS EPVG IIYN PVEY AWEP HRNY VTRY CQGP KEVL FLGM NPGP FGMA QTGV PFGE VSMV RDWL GIVG PVLT PPQE HPKR PVLG LECP QSEV SGAR FWGF FRNL CGQP EVFF HHCF VHNL CPLL FLAP SGRN LTPA ELPA KQRE QLLG ICDA ALCR QVQL LGVR LVVG VGRL AEQR ARRA LAGL MPEV QVEG LLHP SPRN PQAN KGWE AVAK ERLN ELGL LPLL LK
Gene ID:	23583
Uniprot:	Q53HV7
Cellular Location:	Nucleus
Calculated MW:	19kDa/29kDa
Observed MW:	30kDa

Synonyms:	SMUG1, FDG, HMUDG, UNG3
Background:	This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants.

UniProt Protein Function:	SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.2.2.-; Hydrolase; DNA repair, damage Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: nucleolus; nucleoplasm Molecular Function:DNA N-glycosylase activity; oxidized pyrimidine base lesion DNA N-glycosylase activity; protein binding; single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity Biological Process: base-excision repair; depyrimidination
NCBI Summary:	This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]
UniProt Code:	Q53HV7
NCBI GenInfo Identifier:	116242793
NCBI Gene ID:	23583
NCBI Accession:	Q53HV7.2
UniProt Secondary Accession:	Q53HV7,O95862, Q0D2M0, Q8NB71, Q9BWC8, A8K2K9,
UniProt Related Accession:	Q53HV7
Molecular Weight:	19,627 Da
NCBI Full Name:	Single-strand selective monofunctional uracil DNA glycosylase
NCBI Synonym Full Names:	single-strand-selective monofunctional uracil-DNA glycosylase 1
NCBI Official Symbol:	SMUG1
NCBI Official Synonym Symbols:	FDG; UNG3; HMUDG
NCBI Protein Information:	single-strand selective monofunctional uracil DNA glycosylase
UniProt Protein Name:	Single-strand selective monofunctional uracil DNA glycosylase
Protein Family:	Single-strand selective monofunctional uracil DNA glycosylase
UniProt Gene Name:	SMUG1
UniProt Entry Name:	SMUG1_HUMAN

Western blot analysis of extracts of various cell lines, using SMUG1 antibody (CAB10166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

Antibodies

SMUG1 Antibody (PACO03722)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)