Cell Biology Antibodies 10

Anti-SMTN Antibody (CAB6745)

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SKU:
CAB6745
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SMTN Antibody
Antibody SKU:CAB6745
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-325 of human SMTN (NP_001193946.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 5637, Mouse kidney, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-325 of human SMTN (NP_001193946.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EGAL RKLL EVTA DLAE RRRI RSAI RELQ RQEL EREE EALA SKRF RAER QDNK ENWL HSQQ REAE QRAA LARL AGQL ESMN DVEE LTAL LRSA GEYE ERKL IRAA IRRV RAQE IEAA TLAG RLYS GRPN SGSR EDSK GLAA HRLE QCEV PERE EQEQ QAEV SKPT PTPE GTSQ DVTT VTLL LRAP PGST SSSP ASPS SSPT PASP EPPL EPAE AQCL TAEV PGSP EPPP SPPK TTSP EPQE SPTL PSTE GQVV NKLL SGPK E
Gene ID:6525
Uniprot:P53814
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:50kDa/98kDa/99kDa/101kDa
Observed MW:117kDa
Synonyms:SMTN
Background:This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:smoothelin: Structural protein of the cytoskeleton. Belongs to the smoothelin family. 4 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; actin cytoskeleton

Molecular Function:structural constituent of muscle; actin binding

Biological Process: muscle development; smooth muscle contraction

NCBI Summary:This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2011]
UniProt Code:P53814
NCBI GenInfo Identifier:338817991
NCBI Gene ID:6525
NCBI Accession:P53814.7
UniProt Secondary Accession:P53814,O00569, O95769, O95937, Q8N4H8, Q8WWW1, Q8WWW2 Q9P1S8, Q9UIT1, Q9UIT2,
UniProt Related Accession:P53814
Molecular Weight:917
NCBI Full Name:Smoothelin
NCBI Synonym Full Names:smoothelin
NCBI Official Symbol:SMTN  
NCBI Protein Information:smoothelin
UniProt Protein Name:Smoothelin
Protein Family:Smoothelin
UniProt Gene Name:SMTN  
UniProt Entry Name:SMTN_HUMAN
Anti-SMTN Antibody (CAB6745)Western blot analysis of extracts of various cell lines, using SMTN antibody (CAB6745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.
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