Cell Cycle Antibodies 1

Anti-SMPD3 Antibody (CAB10197)

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SKU:
CAB10197
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SMPD3 Antibody
Antibody SKU:CAB10197
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 416-655 of human SMPD3 (NP_061137.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:3000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, LO2, A-549, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 416-655 of human SMPD3 (NP_061137.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDVA YHCY PNKC NDDA LASK GALF LKVQ VGST PQDQ RIVG YIAC THLH APQE DSAI RCGQ LDLL QDWL ADFR KSTS SSSA ANPE ELVA FDVV CGDF NFDN CSSD DKLE QQHS LFTH YRDP CRLG PGEE KPWA IGTL LDTN GLYD EDVC TPDN LQKV LESE EGRR EYLA FPTS KSSG QKGR KELL KGNG RRID YMLH AEEG LCPD WKAE VEEF SFIT QLSG LTDH LPVA MRLM VSSG EEEA
Gene ID:55512
Uniprot:Q9NY59
Cellular Location:Cell membrane, Golgi apparatus membrane, Lipid-anchor
Calculated MW:70kDa/71kDa
Observed MW:80kDa
Synonyms:SMPD3, NSMASE2
Background:
UniProt Protein Function:SMPD3: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Belongs to the neutral sphingomyelinase family.
UniProt Protein Details:

Protein type:Lipid Metabolism - sphingolipid; Phospholipase; Membrane protein, integral; EC 3.1.4.12

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: plasma membrane

Biological Process: glycosphingolipid metabolic process; sphingomyelin catabolic process

UniProt Code:Q9NY59
NCBI GenInfo Identifier:73921262
NCBI Gene ID:55512
NCBI Accession:Q9NY59.1
UniProt Secondary Accession:Q9NY59,Q2M1S8, B7ZL82,
UniProt Related Accession:Q9NY59
Molecular Weight:70,170 Da
NCBI Full Name:Sphingomyelin phosphodiesterase 3
NCBI Synonym Full Names:sphingomyelin phosphodiesterase 3
NCBI Official Symbol:SMPD3  
NCBI Official Synonym Symbols:NSMASE2  
NCBI Protein Information:sphingomyelin phosphodiesterase 3
UniProt Protein Name:Sphingomyelin phosphodiesterase 3
UniProt Synonym Protein Names:Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II
Protein Family:Sphingomyelin phosphodiesterase
UniProt Gene Name:SMPD3  
UniProt Entry Name:NSMA2_HUMAN
Anti-SMPD3 Antibody (CAB10197)Western blot analysis of extracts of various cell lines, using SMPD3 antibody (CAB10197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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