Cell Cycle Antibodies 1
Anti-SMPD3 Antibody (CAB10197)
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- SKU:
- CAB10197
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-SMPD3 Antibody |
| Antibody SKU: | CAB10197 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 416-655 of human SMPD3 (NP_061137.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:3000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, LO2, A-549, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 416-655 of human SMPD3 (NP_061137.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDVA YHCY PNKC NDDA LASK GALF LKVQ VGST PQDQ RIVG YIAC THLH APQE DSAI RCGQ LDLL QDWL ADFR KSTS SSSA ANPE ELVA FDVV CGDF NFDN CSSD DKLE QQHS LFTH YRDP CRLG PGEE KPWA IGTL LDTN GLYD EDVC TPDN LQKV LESE EGRR EYLA FPTS KSSG QKGR KELL KGNG RRID YMLH AEEG LCPD WKAE VEEF SFIT QLSG LTDH LPVA MRLM VSSG EEEA |
| Gene ID: | 55512 |
| Uniprot: | Q9NY59 |
| Cellular Location: | Cell membrane, Golgi apparatus membrane, Lipid-anchor |
| Calculated MW: | 70kDa/71kDa |
| Observed MW: | 80kDa |
| Synonyms: | SMPD3, NSMASE2 |
| Background: |
| UniProt Protein Function: | SMPD3: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Belongs to the neutral sphingomyelinase family. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - sphingolipid; Phospholipase; Membrane protein, integral; EC 3.1.4.12 Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: plasma membrane Biological Process: glycosphingolipid metabolic process; sphingomyelin catabolic process |
| UniProt Code: | Q9NY59 |
| NCBI GenInfo Identifier: | 73921262 |
| NCBI Gene ID: | 55512 |
| NCBI Accession: | Q9NY59.1 |
| UniProt Secondary Accession: | Q9NY59,Q2M1S8, B7ZL82, |
| UniProt Related Accession: | Q9NY59 |
| Molecular Weight: | 70,170 Da |
| NCBI Full Name: | Sphingomyelin phosphodiesterase 3 |
| NCBI Synonym Full Names: | sphingomyelin phosphodiesterase 3 |
| NCBI Official Symbol: | SMPD3 |
| NCBI Official Synonym Symbols: | NSMASE2 |
| NCBI Protein Information: | sphingomyelin phosphodiesterase 3 |
| UniProt Protein Name: | Sphingomyelin phosphodiesterase 3 |
| UniProt Synonym Protein Names: | Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II |
| Protein Family: | Sphingomyelin phosphodiesterase |
| UniProt Gene Name: | SMPD3 |
| UniProt Entry Name: | NSMA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SMPD3 antibody (CAB10197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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