Name: Anti-SMPD2 Antibody (CAB1166)
Brand: Metabolism Antibodies 1
SKU: CAB1166
Availability: OutOfStock

Antibody Name:	Anti-SMPD2 Antibody
Antibody SKU:	CAB1166
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMPD2 (NP_003071.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMPD2 (NP_003071.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AHRV AQAW ELAQ FIHH TSKK ADVV LLCG DLNM HPED LGCC LLKE WTGL HDAY LETR DFKG SEEG NTMV PKNC YVSQ QELK PFPF GVRI DYVL YKAV SGFY I
Gene ID:	6610
Uniprot:	O60906
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	47kDa
Observed MW:	50kDa

Synonyms:	SMPD2, ISC1, NSMASE, NSMASE1
Background:	This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase.

UniProt Protein Function:	SMPD2: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. Belongs to the neutral sphingomyelinase family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Phosphodiesterase; Lipid Metabolism - sphingolipid; Membrane protein, multi-pass; EC 3.1.4.12 Chromosomal Location of Human Ortholog: 6q21 Cellular Component: integral to plasma membrane; plasma membrane; caveola Molecular Function:sphingomyelin phosphodiesterase activity; metal ion binding Biological Process: sphingolipid metabolic process; sphingomyelin metabolic process; nerve growth factor receptor signaling pathway; response to mechanical stimulus; ceramide biosynthetic process; glycosphingolipid metabolic process
NCBI Summary:	This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009]
UniProt Code:	O60906
NCBI GenInfo Identifier:	160332206
NCBI Gene ID:	6610
NCBI Accession:	O60906.2
UniProt Related Accession:	O60906
Molecular Weight:	48kDa
NCBI Full Name:	Sphingomyelin phosphodiesterase 2
NCBI Synonym Full Names:	sphingomyelin phosphodiesterase 2
NCBI Official Symbol:	SMPD2
NCBI Official Synonym Symbols:	ISC1; NSMASE; NSMASE1
NCBI Protein Information:	sphingomyelin phosphodiesterase 2
UniProt Protein Name:	Sphingomyelin phosphodiesterase 2
UniProt Synonym Protein Names:	Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase
UniProt Gene Name:	SMPD2
UniProt Entry Name:	NSMA_HUMAN

Western blot analysis of extracts of various cell lines, using SMPD2 antibody (CAB1166) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)