Metabolism Antibodies 1

Anti-SMPD2 Antibody (CAB1166)

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SKU:
CAB1166
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-SMPD2 Antibody
Antibody SKU:CAB1166
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMPD2 (NP_003071.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMPD2 (NP_003071.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AHRV AQAW ELAQ FIHH TSKK ADVV LLCG DLNM HPED LGCC LLKE WTGL HDAY LETR DFKG SEEG NTMV PKNC YVSQ QELK PFPF GVRI DYVL YKAV SGFY I
Gene ID:6610
Uniprot:O60906
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:47kDa
Observed MW:50kDa
Synonyms:SMPD2, ISC1, NSMASE, NSMASE1
Background:This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase.
UniProt Protein Function:SMPD2: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2- lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso- sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. Belongs to the neutral sphingomyelinase family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Phosphodiesterase; Lipid Metabolism - sphingolipid; Membrane protein, multi-pass; EC 3.1.4.12

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: integral to plasma membrane; plasma membrane; caveola

Molecular Function:sphingomyelin phosphodiesterase activity; metal ion binding

Biological Process: sphingolipid metabolic process; sphingomyelin metabolic process; nerve growth factor receptor signaling pathway; response to mechanical stimulus; ceramide biosynthetic process; glycosphingolipid metabolic process

NCBI Summary:This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009]
UniProt Code:O60906
NCBI GenInfo Identifier:160332206
NCBI Gene ID:6610
NCBI Accession:O60906.2
UniProt Related Accession:O60906
Molecular Weight:48kDa
NCBI Full Name:Sphingomyelin phosphodiesterase 2
NCBI Synonym Full Names:sphingomyelin phosphodiesterase 2
NCBI Official Symbol:SMPD2  
NCBI Official Synonym Symbols:ISC1; NSMASE; NSMASE1  
NCBI Protein Information:sphingomyelin phosphodiesterase 2
UniProt Protein Name:Sphingomyelin phosphodiesterase 2
UniProt Synonym Protein Names:Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase
UniProt Gene Name:SMPD2  
UniProt Entry Name:NSMA_HUMAN
Anti-SMPD2 Antibody (CAB1166)Western blot analysis of extracts of various cell lines, using SMPD2 antibody (CAB1166) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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