Cell Biology Antibodies 10
Anti-SMPD1 / ASM Antibody (CAB6743)
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- SKU:
- CAB6743
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SMPD1 / ASM Antibody |
| Antibody SKU: | CAB6743 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse heart, Mouse lung, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PARL HRIV PRLR DVFG WGNL TCPI CKGL FTAI NLGL KKEP NVAR VGSV AIKL CNLL KIAP PAVC QSIV HLFE DDMV EVWR RSVL SPSE ACGL LLGS TCGH WDIF SSWN ISLP TVPK PPPK PPSP PAPG APVS RILF LTDL HWDH DYLE GTDP DCAD PLCC RRGS GLPP ASRP GAGY WGEY SKCD LPLR TLES LLSG LGPA GPFD MVYW TGDI PAHD VWHQ TRQD QLRA LTTV TALV RKFL GPVP VYPA VGNH ESTP VNSF PPPF IEGN HSS |
| Gene ID: | 6609 |
| Uniprot: | P17405 |
| Cellular Location: | Lysosome |
| Calculated MW: | 63kDa/64kDa/69kDa |
| Observed MW: | 60kDa |
| Synonyms: | SMPD1, ASM, ASMASE, NPD, SMPD1/ASM |
| Background: | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Phosphodiesterase; Lipid Metabolism - sphingolipid; EC 3.1.4.12 Chromosomal Location of Human Ortholog: 11p15.4-p15.1 Cellular Component: endosome; extracellular space; lysosomal lumen; lysosome; plasma membrane Molecular Function:protein binding; sphingomyelin phosphodiesterase activity; zinc ion binding Biological Process: ceramide biosynthetic process; glycosphingolipid metabolic process; negative regulation of MAP kinase activity; nervous system development; positive regulation of protein amino acid dephosphorylation; signal transduction; sphingomyelin catabolic process; sphingomyelin metabolic process Disease: Niemann-pick Disease, Type A; Niemann-pick Disease, Type B |
| NCBI Summary: | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P17405 |
| NCBI GenInfo Identifier: | 224471897 |
| NCBI Gene ID: | 6609 |
| NCBI Accession: | P17405.4 |
| UniProt Secondary Accession: | P17405,P17406, Q13811, Q16837, Q16841, A8K8M3, E9PKS3 |
| UniProt Related Accession: | P17405 |
| Molecular Weight: | 69,624 Da |
| NCBI Full Name: | Sphingomyelin phosphodiesterase |
| NCBI Synonym Full Names: | sphingomyelin phosphodiesterase 1 |
| NCBI Official Symbol: | SMPD1 |
| NCBI Official Synonym Symbols: | ASM; NPD; ASMASE |
| NCBI Protein Information: | sphingomyelin phosphodiesterase |
| UniProt Protein Name: | Sphingomyelin phosphodiesterase |
| UniProt Synonym Protein Names: | Acid sphingomyelinase; aSMase |
| Protein Family: | Sphingomyelin phosphodiesterase |
| UniProt Gene Name: | SMPD1 |
| UniProt Entry Name: | ASM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SMPD1 / ASM antibody (CAB6743) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using SMPD1 / ASM antibody (CAB6743) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using SMPD1 / ASM antibody (CAB6743). Blue: DAPI for nuclear staining. |
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