Cell Biology Antibodies 6
Anti-SMPD1 / ASM Antibody (CAB16263)
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- SKU:
- CAB16263
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SMPD1 / ASM Antibody |
| Antibody SKU: | CAB16263 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human SMPD1 / ASM |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, HepG2, U-251MG, MCF-7, Mouse brain, Mouse liver, Rat brain, Rat liver |
| Immunogen: | Recombinant protein of human SMPD1 / ASM |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6609 |
| Uniprot: | P17405 |
| Cellular Location: | Lysosome |
| Calculated MW: | 63kDa/64kDa/69kDa |
| Observed MW: | 60kDa |
| Synonyms: | SMPD1, ASM, ASMASE, NPD, SMPD1/ASM |
| Background: | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Phosphodiesterase; Lipid Metabolism - sphingolipid; EC 3.1.4.12 Chromosomal Location of Human Ortholog: 11p15.4-p15.1 Cellular Component: endosome; extracellular space; lysosomal lumen; lysosome; plasma membrane Molecular Function:protein binding; sphingomyelin phosphodiesterase activity; zinc ion binding Biological Process: ceramide biosynthetic process; glycosphingolipid metabolic process; negative regulation of MAP kinase activity; nervous system development; positive regulation of protein amino acid dephosphorylation; signal transduction; sphingomyelin catabolic process; sphingomyelin metabolic process Disease: Niemann-pick Disease, Type A; Niemann-pick Disease, Type B |
| NCBI Summary: | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P17405 |
| NCBI GenInfo Identifier: | 224471897 |
| NCBI Gene ID: | 6609 |
| NCBI Accession: | P17405.4 |
| UniProt Secondary Accession: | P17405,P17406, Q13811, Q16837, Q16841, A8K8M3, E9PKS3 |
| UniProt Related Accession: | P17405 |
| Molecular Weight: | 69,624 Da |
| NCBI Full Name: | Sphingomyelin phosphodiesterase |
| NCBI Synonym Full Names: | sphingomyelin phosphodiesterase 1 |
| NCBI Official Symbol: | SMPD1 |
| NCBI Official Synonym Symbols: | ASM; NPD; ASMASE |
| NCBI Protein Information: | sphingomyelin phosphodiesterase |
| UniProt Protein Name: | Sphingomyelin phosphodiesterase |
| UniProt Synonym Protein Names: | Acid sphingomyelinase; aSMase |
| Protein Family: | Sphingomyelin phosphodiesterase |
| UniProt Gene Name: | SMPD1 |
| UniProt Entry Name: | ASM_HUMAN |
 | Western blot analysis of extracts of THP-1 cells, using SMPD1 / ASM antibody (CAB16263) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of various cell lines, using SMPD1 / ASM antibody (CAB16263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of NIH-3T3 cells using SMPD1 / ASM Polyclonal Antibody (CAB16263) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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