Epigenetics & Nuclear Signaling Antibodies 2

Anti-SMG7 Antibody (CAB15371)

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SKU:
CAB15371
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SMG7 Antibody
Antibody SKU:CAB15371
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMG7 (NP_963863.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMG7 (NP_963863.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSLQ SAQY LRQA EVLK ADMT DSKL GPAE VWTS RQAL QDLY QKML VTDL EYAL DKKV EQDL WNHA FKNQ ITTL QGQA KNRA NPNR SEVQ ANLS LFLE AASG FYTQ LLQE LCTV FNVD LPCR VKSS QLGI ISNK QTHT SAIV KPQS SSCS YICQ HCLV HLGD IARY RNQT SQAE SYYR HAAQ LVPS NGQP YNQL AILA SSKG DHLT TIFY YCRS IAVK FPFP AAST NLQK ALSK ALES RDEV KTKW GVSD FIKA FIKF HGHV YLSK SLEK LSPL REKL EEQF KRLL FQKA FNSQ QLVH VTVI
Gene ID:9887
Uniprot:Q92540
Cellular Location:Cytoplasm, Nucleus
Calculated MW:122kDa/127kDa/131kDa
Observed MW:127kDa
Synonyms:SMG7, C1orf16, EST1C, SGA56M
Background:This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.
NCBI Summary:This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]
UniProt Code:Q92540
NCBI GenInfo Identifier:84028262
NCBI Gene ID:9887
NCBI Accession:Q92540.2
UniProt Secondary Accession:Q92540,Q5T1Q0, Q6PIE0, Q7Z7H9, Q8IXC1, Q8IXC2, B4DRB2 E9PCI0, E9PEH2,
UniProt Related Accession:Q92540
Molecular Weight:127,854 Da
NCBI Full Name:Protein SMG7
NCBI Synonym Full Names:SMG7, nonsense mediated mRNA decay factor
NCBI Official Symbol:SMG7  
NCBI Official Synonym Symbols:EST1C; SGA56M; C1orf16  
NCBI Protein Information:protein SMG7
UniProt Protein Name:Protein SMG7
UniProt Synonym Protein Names:EST1-like protein C; SMG-7 homolog; hSMG-7
Protein Family:Protein
UniProt Gene Name:SMG7  
UniProt Entry Name:SMG7_HUMAN
Anti-SMG7 Antibody (CAB15371)Western blot analysis of extracts of various cell lines, using SMG7 antibody (CAB15371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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