Epigenetics & Nuclear Signaling Antibodies 4

Anti-SMCHD1 Antibody (CAB7214)

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SKU:
CAB7214
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SMCHD1 Antibody
Antibody SKU:CAB7214
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1756-2005 of human SMCHD1 (NP_056110.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Rat
Positive Samples:HL-60, SKOV3, Jurkat, U-251MG, HepG2, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1756-2005 of human SMCHD1 (NP_056110.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TTDA ARRI YDET QGRQ QVLP LDSI YKKT LPDW KRSL PHFR NGKL YFKP IGDP VFAR DLLT FPDN VEHC ETVF GMLL GDTI ILDN LDAA NHYR KEVV KITH CPTL LTRD GDRI RSNG KFGG LQNK APPM DKLR GMVF GAPV PKQC LILG EQID LLQQ YRSA VCKL DSVN KDLN SQLE YLRT PDMR KKKQ ELDE HEKN LKLI EEKL GMTP IRKC NDSL RHSP KVET TDCP VPPK RMRR EATR QNRI ITKT DV
Gene ID:23347
Uniprot:A6NHR9
Cellular Location:Chromosome
Calculated MW:85kDa/215kDa/226kDa
Observed MW:246kDa
Synonyms:SMCHD1, BAMS, FSHD2
Background:This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family.
UniProt Protein Function:SMCHD1: Required for maintenance of X inactivation in females and hypermethylation of CpG islands associated with inactive X. Involved in a pathway that mediates the methylation of a subset of CpG islands slowly and requires the de novo methyltransferase DNMT3B. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: Barr body

Molecular Function:ATP binding

Biological Process: chromosome organization and biogenesis

Disease: Facioscapulohumeral Muscular Dystrophy 2

NCBI Summary:This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family. [provided by RefSeq, Dec 2011]
UniProt Code:A6NHR9
NCBI GenInfo Identifier:187611512
NCBI Gene ID:23347
NCBI Accession:A6NHR9.2
UniProt Secondary Accession:A6NHR9,O75141, Q6AHX6, Q6ZTQ8, Q9H6Q2, Q9UG39,
UniProt Related Accession:A6NHR9
Molecular Weight:2005
NCBI Full Name:Structural maintenance of chromosomes flexible hinge domain-containing protein 1
NCBI Synonym Full Names:structural maintenance of chromosomes flexible hinge domain containing 1
NCBI Official Symbol:SMCHD1  
NCBI Protein Information:structural maintenance of chromosomes flexible hinge domain-containing protein 1; SMC hinge domain-containing protein 1
UniProt Protein Name:Structural maintenance of chromosomes flexible hinge domain-containing protein 1
UniProt Synonym Protein Names:SMC hinge domain-containing protein 1
Protein Family:Structural maintenance of chromosomes flexible hinge domain-containing protein
UniProt Gene Name:SMCHD1  
UniProt Entry Name:SMHD1_HUMAN
Anti-SMCHD1 Antibody (CAB7214)Western blot analysis of extracts of various cell lines, using SMCHD1 antibody (CAB7214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-SMCHD1 Antibody (CAB7214)Immunofluorescence analysis of MCF-7 cells using SMCHD1 antibody (CAB7214). Blue: DAPI for nuclear staining.
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