Cell Cycle Antibodies 1

Anti-SMC5 Antibody (CAB2301)

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SKU:
CAB2301
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SMC5 Antibody
Antibody SKU:CAB2301
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 872-1101 of human SMC5 (NP_055925.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:3000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, HeLa, Jurkat, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 872-1101 of human SMC5 (NP_055925.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LTEE RSRA SCFT GLNP TIVQ EYTK REEE IEQL TEEL KGKK VELD QYRE NISQ VKER WLNP LKEL VEKI NEKF SNFF SSMQ CAGE VDLH TENE EDYD KYGI RIRV KFRS STQL HELT PHHQ SGGE RSVS TMLY LMAL QELN RCPF RVVD EINQ GMDP INER RVFE MVVN TACK ENTS QYFF ITPK LLQN LPYS EKMT VLFV YNGP HMLE PNTW NLKA FQRR RRRI TFTQ PS
Gene ID:23137
Uniprot:Q8IY18
Cellular Location:Chromosome, Nucleus, PML body, telomere
Calculated MW:128kDa
Observed MW:132kDa
Synonyms:SMC5, SMC5L1
Background:
UniProt Protein Function:SMC5: Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. Belongs to the SMC family. SMC5 subfamily.
UniProt Protein Details:

Protein type:DNA replication

Chromosomal Location of Human Ortholog: 9q21.12

Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; cell junction; nucleus

Molecular Function:protein binding; ATP binding

Biological Process: mitosis; positive regulation of maintenance of mitotic sister chromatid cohesion; cell division; telomere maintenance via recombination; positive regulation of mitotic metaphase/anaphase transition; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination

UniProt Code:Q8IY18
NCBI GenInfo Identifier:122070387
NCBI Gene ID:23137
NCBI Accession:Q8IY18.2
UniProt Related Accession:Q8IY18
Molecular Weight:
NCBI Full Name:Structural maintenance of chromosomes protein 5
NCBI Synonym Full Names:structural maintenance of chromosomes 5
NCBI Official Symbol:SMC5  
NCBI Official Synonym Symbols:SMC5L1  
NCBI Protein Information:structural maintenance of chromosomes protein 5
UniProt Protein Name:Structural maintenance of chromosomes protein 5
Protein Family:SMC5-SMC6 complex localization factor protein
UniProt Gene Name:SMC5  
UniProt Entry Name:SMC5_HUMAN
Anti-SMC5 Antibody (CAB2301)Western blot analysis of extracts of various cell lines, using SMC5 antibody (CAB2301) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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