Cell Cycle Antibodies 1

Anti-SMC2 Antibody (CAB17867)

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SKU:
CAB17867
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SMC2 Antibody
Antibody SKU:CAB17867
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 700-900 of human SMC2 (NP_006435.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:MCF7, Raji, U-87MG, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 700-900 of human SMC2 (NP_006435.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EELA GLKN TAEK YRQL KQQW EMKT EEAD LLQT KLQQ SSYH KQQE ELDA LKKT IEES EETL KNTK EIQR KAEE KYEV LENK MKNA EAER EREL KDAQ KKLD CAKT KADA SSKK MKEK QQEV EAIT LELE ELKR EHTS YKQQ LEAV NEAI KSYE SQIE VMAA EVAK NKES VNKA QEEV TKQK EVIT AQDT VIKA KYAE VAKH K
Gene ID:10592
Uniprot:O95347
Cellular Location:Chromosome, Cytoplasm, Nucleus
Calculated MW:124kDa/135kDa
Observed MW:135kDa
Synonyms:SMC2, CAP-E, CAPE, SMC-2, SMC2L1
Background:
UniProt Protein Function:SMC2L1: Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Forms an heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. Belongs to the SMC family. SMC2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: condensed chromosome; condensin complex; cytoplasm; cytosol; nuclear chromosome; nucleolus; nucleoplasm; nucleus

Molecular Function:protein binding; protein heterodimerization activity

Biological Process: mitotic chromosome condensation

UniProt Code:O95347
NCBI GenInfo Identifier:215273886
NCBI Gene ID:10592
NCBI Accession:O95347.2
UniProt Secondary Accession:O95347,Q6IEE0, Q9P1P2,
UniProt Related Accession:O95347
Molecular Weight:124,926 Da
NCBI Full Name:Structural maintenance of chromosomes protein 2
NCBI Synonym Full Names:structural maintenance of chromosomes 2
NCBI Official Symbol:SMC2  
NCBI Official Synonym Symbols:CAPE; CAP-E; SMC-2; SMC2L1  
NCBI Protein Information:structural maintenance of chromosomes protein 2
UniProt Protein Name:Structural maintenance of chromosomes protein 2
UniProt Synonym Protein Names:Chromosome-associated protein E; hCAP-E; XCAP-E homolog
UniProt Gene Name:SMC2  
UniProt Entry Name:SMC2_HUMAN
Anti-SMC2 Antibody (CAB17867)Western blot analysis of extracts of various cells, using SMC2 antibody (CAB17867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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