Cell Cycle Antibodies 1

Anti-SMC1B Antibody (CAB12905)

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SKU:
CAB12905
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SMC1B Antibody
Antibody SKU:CAB12905
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1012-1161 of human SMC1B (NP_001278430.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Rat
Positive Samples:Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1012-1161 of human SMC1B (NP_001278430.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ILLK TAAP NLRA LENL KTVR DKFQ ESTD AFEA SRKE ARLC RQEF EQVK KRRY DLFT QCFE HVSI SIDQ IYKK LCRN NSAQ VSSY IKEQ TQDQ FQMI VISL KEEF YSRA DALI GIYP EYDD CMFS RVLT LDLS QYPD TEGQ ESSK RHGE SR
Gene ID:27127
Uniprot:Q8NDV3
Cellular Location:Chromosome, Nucleus, centromere
Calculated MW:135kDa/143kDa
Observed MW:144kDa
Synonyms:SMC1B, SMC1BETA, SMC1L2
Background:
UniProt Protein Function:SMC1B: Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. Belongs to the SMC family. SMC1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: cytoplasm; nucleoplasm

NCBI Summary:SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 [PubMed 11564881]).[supplied by OMIM, Mar 2008]
UniProt Code:Q8NDV3
NCBI GenInfo Identifier:57015410
NCBI Gene ID:27127
NCBI Accession:Q8NDV3.2
UniProt Secondary Accession:Q8NDV3,Q5TIC3, Q6ZUF9, Q9Y3G5, A0AV46, B0QY23, B0QY24
UniProt Related Accession:Q8NDV3
Molecular Weight:143,837 Da
NCBI Full Name:Structural maintenance of chromosomes protein 1B
NCBI Synonym Full Names:structural maintenance of chromosomes 1B
NCBI Official Symbol:SMC1B  
NCBI Official Synonym Symbols:SMC1L2; SMC1BETA  
NCBI Protein Information:structural maintenance of chromosomes protein 1B
UniProt Protein Name:Structural maintenance of chromosomes protein 1B
Protein Family:Structural maintenance of chromosomes protein
UniProt Gene Name:SMC1B  
UniProt Entry Name:SMC1B_HUMAN
Anti-SMC1B Antibody (CAB12905)Western blot analysis of extracts of rat testis, using SMC1B antibody (CAB12905) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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