Antibody Name:	Anti-SMARCE1 Antibody [KO Validated]
Antibody SKU:	CAB13353
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, MCF-7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKR PSYA PPPT PAPA TQMP STPG FVGY NPYS HLAY NNYR LGGN PGTN SRVT ASSG ITIP KPPK PPDK PLMP YMRY SRKV WDQV KASN PDLK LWEI GKII GGMW RDLT DEEK QEYL NEYE AEKI EYNE SMKA YHNS PAYL AYIN AKSR AEAA LEEE SRQR QSRM EKGE PYMS IQPA EDPD DYDD GFSM KHTA TARF QRNH RLIS EILS ESVV PDVR SVVT TARM QVLK RQVQ SLMV HQRK LEAE LLQI EERH QEKK RKFL ESTD SFNN ELKR LCGL KVEV DMEK IAAE IAQA EEQA RKRQ EERE KEAA EQAE RSQS SIVP EEEQ AANK GEEK KDDE NIPM ETEE THLE ETTE SQQN GEEG TSTP EDKE SGQE GVDS MAEE GTSD SNTG SESN SATV EEPP TDPI PEDE KKE
Gene ID:	6605
Uniprot:	Q969G3
Cellular Location:	Nucleus
Calculated MW:	34kDa/37kDa/39kDa/41kDa/42kDa/46kDa
Observed MW:	55kDa

Synonyms:

SMARCE1, BAF57, CSS5

Background:

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

UniProt Protein Function:	SMARCE1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCE1 may be a cause of Coffin-Siris syndrome, a highly variable disease characterized by mental retardation associated with a broad spectrum of different clinical features. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: nucleoplasm; nuclear chromosome; SWI/SNF complex; protein complex; transcriptional repressor complex; nuclear chromatin; nucleus Molecular Function:ligand-dependent nuclear receptor binding; N-acetyltransferase activity; protein binding; nucleosomal DNA binding; RNA binding; transcription coactivator activity; protein N-terminus binding; chromatin binding Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nervous system development; nucleosome disassembly; metabolic process; ATP-dependent chromatin remodeling; negative regulation of transcription, DNA-dependent Disease: Meningioma, Familial, Susceptibility To
NCBI Summary:	The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]
UniProt Code:	Q969G3
NCBI GenInfo Identifier:	61247587
NCBI Gene ID:	6605
NCBI Accession:	Q969G3.2
UniProt Secondary Accession:	Q969G3,O43539, B3KMC1, B4DFR4, C0IMW4, C0IMW5, C0IMW7 H7C3F6,
UniProt Related Accession:	Q969G3
Molecular Weight:	411
NCBI Full Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
NCBI Synonym Full Names:	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
NCBI Official Symbol:	SMARCE1
NCBI Official Synonym Symbols:	BAF57
NCBI Protein Information:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BRG1-associated factor 57; chromatin remodeling complex BRG1-associated factor 57; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
UniProt Protein Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
UniProt Synonym Protein Names:	BRG1-associated factor 57; BAF57
Protein Family:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
UniProt Gene Name:	SMARCE1
UniProt Entry Name:	SMCE1_HUMAN

	Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (CAB13353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (CAB13353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (CAB13353).
	Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (CAB13353). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (CAB13353) at a dilition of 1:1000.