Neuroscience

Anti-SMARCC1/BAF155 Antibody (CAB4275)

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SKU:
CAB4275
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Neuroscience

Description

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Antibody Name:Anti-SMARCC1/BAF155 Antibody
Antibody SKU:CAB4275
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human SMARCC1/BAF155
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, HepG2, Mouse brain, Mouse testis
Immunogen:A synthesized peptide derived from human SMARCC1/BAF155
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6599
Uniprot:Q92922
Cellular Location:
Calculated MW:155kDa
Observed MW:155KDa
Synonyms:BAF155, CRACC1, Rsc8, SRG3, SWI3
Background:The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]
UniProt Protein Function:SMARCC1: a core component of the BAF (SWI/SNF) complex. This family of complexes remodels chromatin structures, enabling transcription factors to gain access to DNA. They play important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, BAF53A or BAF53B, SMARCC2 and SMARCB1. Other subunits specific to each of the complexes may also be present. Component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. The minimal complex composed of SMARCC1 and SMARCA4 seems to be able to associate with cyclin such as CCNE1 or transcription factors such as KLF1 or GATA1. Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. Belongs to the SMARCC family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nuclear chromatin; nucleoplasm; protein complex; SWI/SNF complex

Molecular Function:nucleosomal DNA binding; protein binding; protein N-terminus binding

Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; nucleosome disassembly; positive regulation of transcription, DNA-dependent

NCBI Summary:The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]
UniProt Code:Q92922
NCBI GenInfo Identifier:209572723
NCBI Gene ID:6599
NCBI Accession:Q92922.3
UniProt Secondary Accession:Q92922,Q17RS0, Q6P172, Q8IWH2,
UniProt Related Accession:Q92922
Molecular Weight:122,867 Da
NCBI Full Name:SWI/SNF complex subunit SMARCC1
NCBI Synonym Full Names:SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1
NCBI Official Symbol:SMARCC1  
NCBI Official Synonym Symbols:Rsc8; SRG3; SWI3; BAF155; CRACC1  
NCBI Protein Information:SWI/SNF complex subunit SMARCC1
UniProt Protein Name:SWI/SNF complex subunit SMARCC1
UniProt Synonym Protein Names:BRG1-associated factor 155; BAF155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1
Protein Family:SWI/SNF complex
UniProt Gene Name:SMARCC1  
UniProt Entry Name:SMRC1_HUMAN
Western blot - SMARCC1/BAF155 Rabbit mAb Western blot analysis of extracts of various cell lines, using SMARCC1/BAF155 Rabbit mAb at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunofluorescence - SMARCC1/BAF155 Rabbit mAb Immunofluorescence analysis of NIH-3T3 cells using SMARCC1/BAF155 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation - SMARCC1/BAF155 Rabbit mAb Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug SMARCC1/BAF155 antibody at a dilition of 1:500.
Chromatin Immunoprecipitation - SMARCC1/BAF155 Rabbit mAb Chromatin immunoprecipitation analysis of extracts of MCF7 beta-estradiol cells, using SMARCC1/BAF155 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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