Name: Anti-SMAD9 Antibody (CAB7518)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB7518
Availability: OutOfStock

Antibody Name:	Anti-SMAD9 Antibody
Antibody SKU:	CAB7518
Antibody Size:	20uL, 50uL, 100uL
Application:	IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1).

Application:	IHC IF
Recommended Dilution:	IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, 293T, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MHST TPIS SLFS FTSP AVKR LLGW KQGD EEEK WAEK AVDS LVKK LKKK KGAM DELE RALS CPGQ PSKC VTIP RSLD GRLQ VSHR KGLP HVIY CRVW RWPD LQSH HELK PLEC CEFP FGSK QKEV CINP YHYR RVET PVLP PVLV PRHS EYNP QLSL LAKF RSAS LHSE PLMP HNAT YPDS FQQP PCSA LPPS PSHA FSQS PCTA SYPH SPGS PSEP ESPY QHSD FRPV CYEE PQHW CSVA YYEL NNRV GETF QASS RSVL IDGF TDPS NNRN RFCL GLLS NVNR NSTI ENTR RHIG KGVH
Gene ID:	4093
Uniprot:	O15198
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	48kDa/52kDa
Observed MW:	45-52kDa

Synonyms:	SMAD9, MADH6, MADH9, PPH2, SMAD8, SMAD8/9, SMAD8A, SMAD8B
Background:	The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Smad8: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Two alternatively spliced products have been described.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; DNA-binding Chromosomal Location of Human Ortholog: 13q12-q14 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; intracellular; cytosol; nucleus Molecular Function:protein binding; DNA binding; metal ion binding; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity Biological Process: BMP signaling pathway; Mullerian duct regression; transcription, DNA-dependent; ureteric bud development; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; cartilage development; midbrain development; response to hypoxia; positive regulation of cell differentiation; protein amino acid phosphorylation; hindbrain development Disease: Pulmonary Hypertension, Primary, 2
NCBI Summary:	The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]
UniProt Code:	O15198
NCBI GenInfo Identifier:	13959539
NCBI Gene ID:	4093
NCBI Accession:	O15198.1
UniProt Secondary Accession:	O15198,O14989, Q5TBA1, A2A2Y6,
UniProt Related Accession:	O15198
Molecular Weight:	467
NCBI Full Name:	Mothers against decapentaplegic homolog 9
NCBI Synonym Full Names:	SMAD family member 9
NCBI Official Symbol:	SMAD9
NCBI Official Synonym Symbols:	PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
NCBI Protein Information:	mothers against decapentaplegic homolog 9; MAD homolog 9; SMAD, mothers against DPP homolog 9; Mothers against decapentaplegic, drosophila, homolog of, 9
UniProt Protein Name:	Mothers against decapentaplegic homolog 9
UniProt Synonym Protein Names:	Madh6; SMAD family member 9; SMAD 9; Smad9
Protein Family:	Mothers against decapentaplegic
UniProt Gene Name:	SMAD9
UniProt Entry Name:	SMAD9_HUMAN

	Immunohistochemistry of paraffin-embedded human liver damage using SMAD9 antibody (CAB7518) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody (CAB7518) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using SMAD9 antibody (CAB7518) at dilution of 1:100. Blue: DAPI for nuclear staining.