KO Validated Antibodies 2

Anti-Smad5 Antibody [KO Validated] (CAB19117)

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SKU:
CAB19117
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-Smad5 Antibody [KO Validated]
Antibody SKU:CAB19117
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Smad5
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, K-562, C2C12
Immunogen:A synthesized peptide derived from human Smad5
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4090
Uniprot:Q99717
Cellular Location:Cytoplasm, Nucleus
Calculated MW:52kDa
Observed MW:60kDa
Anti-Smad5 Antibody [KO Validated] (CAB19117)Western blot analysis of extracts from normal (control) and Smad5 knockout (KO) 293T cells, using Smad5 antibody (CAB19117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Smad5 Antibody [KO Validated] (CAB19117)Western blot analysis of extracts of K-562 cells, using Smad5 antibody (CAB19117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Smad5 Antibody [KO Validated] (CAB19117)Western blot analysis of extracts of C2C12 cells, using Smad5 antibody (CAB19117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Synonyms:DWFC, JV5-1, MADH5, SMAD5
Background:The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Protein Function:SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; intracellular; nucleus; cytosol

Molecular Function:protein binding; ubiquitin protein ligase binding; metal ion binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: Mullerian duct regression; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; embryonic pattern specification; signal transduction; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; positive regulation of osteoblast differentiation; ureteric bud development; transforming growth factor beta receptor signaling pathway; cartilage development; erythrocyte differentiation; cardiac muscle contraction; osteoblast fate commitment

NCBI Summary:The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:Q99717
NCBI GenInfo Identifier:13959566
NCBI Gene ID:4090
NCBI Accession:Q99717.1
UniProt Secondary Accession:Q99717,O14688, Q15798, Q9UQA1,
UniProt Related Accession:Q99717
Molecular Weight:52,258 Da
NCBI Full Name:Mothers against decapentaplegic homolog 5
NCBI Synonym Full Names:SMAD family member 5
NCBI Official Symbol:SMAD5  
NCBI Official Synonym Symbols:DWFC; JV5-1; MADH5  
NCBI Protein Information:mothers against decapentaplegic homolog 5; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5; MAD, mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5
UniProt Protein Name:Mothers against decapentaplegic homolog 5
UniProt Synonym Protein Names:JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Protein Family:SMAD5 antisense gene protein
UniProt Gene Name:SMAD5  
UniProt Entry Name:SMAD5_HUMAN
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