Epigenetics & Nuclear Signaling Antibodies 1
Anti-Slug Antibody (CAB1057)
- SKU:
- CAB1057
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Slug Antibody |
| Antibody SKU: | CAB1057 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 89-268 of human Slug (NP_003059.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 89-268 of human Slug (NP_003059.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PPPS DTSS KDHS GSES PISD EEER LQSK LSDP HAIE AEKF QCNL CNKT YSTF SGLA KHKQ LHCD AQSR KSFS CKYC DKEY VSLG ALKM HIRT HTLP CVCK ICGK AFSR PWLL QGHI RTHT GEKP FSCP HCNR AFAD RSNL RAHL QTHS DVKK YQCK NCSK TFSR MSLL HKHE ESGC CVAH |
| Gene ID: | 6591 |
| Uniprot: | O43623 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 29kDa |
| Observed MW: | 27kDa |
| Synonyms: | SNAI2, SLUG, SLUGH, SLUGH1, SNAIL2, WS2D |
| Background: | This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. |
| UniProt Protein Function: | Snail2: Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. Belongs to the snail C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; Transcription factor; Apoptosis; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 8q11 Cellular Component: cytoplasm; nuclear chromatin; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; metal ion binding; chromatin binding Biological Process: transcription from RNA polymerase II promoter; substrate-bound cell migration, cell release from substrate; Notch signaling pathway; neural crest cell development; regulation of osteoblast differentiation; negative regulation of chondrocyte differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; osteoblast differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; sensory perception of sound; pigmentation; white fat cell differentiation; positive regulation of fat cell differentiation; epithelial to mesenchymal transition; positive regulation of histone acetylation; negative regulation of cell adhesion mediated by integrin; regulation of chemokine production; positive regulation of cell migration Disease: Waardenburg Syndrome, Type 2d; Piebald Trait |
| NCBI Summary: | This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43623 |
| NCBI GenInfo Identifier: | 11134406 |
| NCBI Gene ID: | 6591 |
| NCBI Accession: | O43623.1 |
| UniProt Related Accession: | O43623 |
| Molecular Weight: | |
| NCBI Full Name: | Zinc finger protein SNAI2 |
| NCBI Synonym Full Names: | snail family transcriptional repressor 2 |
| NCBI Official Symbol: | SNAI2 |
| NCBI Official Synonym Symbols: | SLUG; WS2D; SLUGH; SLUGH1; SNAIL2 |
| NCBI Protein Information: | zinc finger protein SNAI2 |
| UniProt Protein Name: | Zinc finger protein SNAI2 |
| UniProt Synonym Protein Names: | Neural crest transcription factor Slug; Protein snail homolog 2 |
| UniProt Gene Name: | SNAI2 |
| UniProt Entry Name: | SNAI2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Slug antibody (CAB1057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using Slug Antibody (CAB1057) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat spleen using Slug Antibody (CAB1057) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using Slug Antibody (CAB1057) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using Slug antibody (CAB1057). Blue: DAPI for nuclear staining. |
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