Epigenetics & Nuclear Signaling Antibodies 2
Anti-SLIRP Antibody (CAB15524)
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- SKU:
- CAB15524
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-SLIRP Antibody |
| Antibody SKU: | CAB15524 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SLIRP (NP_112487.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, DU145, NIH/3T3, Mouse brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SLIRP (NP_112487.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAS AARG AAAL RRSI NQPV AFVR RIPW TAAS SQLK EHFA QFGH VRRC ILPF DKET GFHR GLGW VQFS SEEG LRNA LQQE NHII DGVK VQVH TRRP KLPQ |
| Gene ID: | 81892 |
| Uniprot: | Q9GZT3 |
| Cellular Location: | Mitochondrion, Nucleus |
| Calculated MW: | 12kDa |
| Observed MW: | 12kDa |
| Synonyms: | SLIRP, C14orf156, DC50, PD04872 |
| Background: |
| UniProt Protein Function: | SLIRP: RNA-binding protein that acts as a nuclear receptor corepressor. Probably acts by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. Binds the STR7 loop of SRA RNA. Also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. |
| UniProt Protein Details: | Protein type:RNA-binding; Nuclear receptor co-regulator; Mitochondrial; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: mitochondrion; perinuclear region of cytoplasm; acrosome; nucleus; ribonucleoprotein complex Molecular Function:nucleotide binding Biological Process: sperm motility; transcription, DNA-dependent; regulation of transcription, DNA-dependent; single fertilization; spermatid development |
| UniProt Code: | Q9GZT3 |
| NCBI GenInfo Identifier: | 13654278 |
| NCBI Gene ID: | |
| NCBI Accession: | NP_112487.1 |
| Molecular Weight: | ~ 12kDa |
| NCBI Full Name: | SRA stem-loop-interacting RNA-binding protein, mitochondrial isoform 1 |
| UniProt Protein Name: | SRA stem-loop-interacting RNA-binding protein, mitochondrial |
| Protein Family: | SRA stem-loop-interacting RNA-binding protein |
| UniProt Gene Name: | SLIRP |
| UniProt Entry Name: | SLIRP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SLIRP antibody (CAB15524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Human colon carcinoma using SLIRP Rabbit pAb (CAB15524) at dilution of 1:100 (40x lens). |
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