Name: Anti-SLC7A5 Antibody (CAB2833)
Brand: Signal Transduction Antibodies 2
SKU: CAB2833
Availability: OutOfStock

Antibody Name:	Anti-SLC7A5 Antibody
Antibody SKU:	CAB2833
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human SLC7A5 (NP_003477.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:1000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-1080

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human SLC7A5 (NP_003477.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGA GPKR RALA APAA EEKE EARE KMLA AKSA DGSA PAGE GEGV TLQR NITL LNGV AIIV GTII GSGI FVTP TGVL KEAG SPGL ALVV WAAC GVFS IVGA LCYA ELGT TISK SGGD YAYM LEVY G
Gene ID:	8140
Uniprot:	Q01650
Cellular Location:	Apical cell membrane, Cytoplasm, Multi-pass membrane protein, cytosol
Calculated MW:	55kDa
Observed MW:	45KDa

Synonyms:	SLC7A5, 4F2LC, CD98, D16S469E, E16, LAT1, MPE16, hLAT1
Background:

UniProt Protein Function:	SLC7A5: Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L- leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
UniProt Protein Details:	Protein type:Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 16q24.3 Cellular Component: membrane; intracellular membrane-bound organelle; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleolus; cytosol; nucleus Molecular Function:L-amino acid transmembrane transporter activity; peptide antigen binding; neutral amino acid transmembrane transporter activity; amino acid transmembrane transporter activity Biological Process: neutral amino acid transport; amino acid metabolic process; nervous system development; transport; amino acid transport; ion transport; cell differentiation; blood coagulation; transmembrane transport; leukocyte migration
UniProt Code:	Q01650
NCBI GenInfo Identifier:	12643412
NCBI Gene ID:	8140
NCBI Accession:	Q01650.2
UniProt Secondary Accession:	Q01650,Q8IV97, Q9UBN8, Q9UP15, Q9UQC0,
UniProt Related Accession:	Q01650
Molecular Weight:	507
NCBI Full Name:	Large neutral amino acids transporter small subunit 1
NCBI Synonym Full Names:	solute carrier family 7 (amino acid transporter light chain, L system), member 5
NCBI Official Symbol:	SLC7A5
NCBI Official Synonym Symbols:	E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
NCBI Protein Information:	large neutral amino acids transporter small subunit 1; 4F2 LC; 4F2 light chain; CD98 light chain; integral membrane protein E16; L-type amino acid transporter 1; solute carrier family 7 member 5; large neutral amino acids transporter 1; y+ system cationic amino acid transporter; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
UniProt Protein Name:	Large neutral amino acids transporter small subunit 1
UniProt Synonym Protein Names:	4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter
Protein Family:	Large neutral amino acids transporter
UniProt Gene Name:	SLC7A5
UniProt Entry Name:	LAT1_HUMAN

Western blot analysis of extracts of HT-1080 cells, using SLC7A5 Rabbit pAb (CAB2833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

SLC7A5 Antibody (PACO12307)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)