Neuroscience

Anti-SLC6A5 Antibody (CAB11647)

(No reviews yet) Write a Review
SKU:
CAB11647
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SLC6A5 Antibody
Antibody SKU:CAB11647
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SLC6A5 (NP_004202.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, U-937, U-87MG, U-251MG, Mouse brain, Mouse liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SLC6A5 (NP_004202.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDCS APKE MNKL PANS PEAA AAQG HPDG PCAP RTSP EQEL PAAA APPP PRVP RSAS TGAQ TFQS ADAR ACEA ERPG VGSC KLSS PRAQ AASA ALRD LREA QGAQ ASPP PGSS GPGN ALHC KIPF LRGP EGDA NVSV GKGT LERN NTPV VGWV NMSQ STVV LGTD GITS VLPG SVAT VATQ EDEQ GDEN KARG NWSS KLDF
Gene ID:9152
Uniprot:Q9Y345
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:87kDa
Observed MW:60kDa
Synonyms:SLC6A5, GLYT-2, GLYT2, HKPX3, NET1
Background:This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission. This protein is found in glycinergic axons and maintains a high presynaptic pool of neurotransmitter at glycinergic synapses. Mutations in this gene cause hyperekplexia; a heterogenous neurological disorder characterized by exaggerated startle responses and neonatal apnea. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SLC6A5: Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses. Defects in SLC6A5 are the cause of hyperekplexia type 3 (HKPX3). HKPX3 is a neurologic disorder characterized by neonatal hypertonia, an exaggerated startle response to tactile or acoustic stimuli, and life- threatening neonatal apnea episodes. Notably, in some cases, symptoms resolved in the first year of life. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A5 subfamily.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function:glycine:sodium symporter activity

Biological Process: synaptic transmission; synaptic transmission, glycinergic; transport

Disease: Hyperekplexia 3

NCBI Summary:This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission. This protein is found in glycinergic axons and maintains a high presynaptic pool of neurotransmitter at glycinergic synapses. Mutations in this gene cause hyperekplexia; a heterogenous neurological disorder characterized by exaggerated startle responses and neonatal apnea. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
UniProt Code:Q9Y345
NCBI GenInfo Identifier:296452967
NCBI Gene ID:9152
NCBI Accession:Q9Y345.3
UniProt Secondary Accession:Q9Y345,O95288, Q4VAM7, Q9BX77,
UniProt Related Accession:Q9Y345
Molecular Weight:87kDa
NCBI Full Name:Sodium- and chloride-dependent glycine transporter 2
NCBI Synonym Full Names:solute carrier family 6 member 5
NCBI Official Symbol:SLC6A5  
NCBI Official Synonym Symbols:NET1; GLYT2; HKPX3; GLYT-2  
NCBI Protein Information:sodium- and chloride-dependent glycine transporter 2
UniProt Protein Name:Sodium- and chloride-dependent glycine transporter 2
UniProt Synonym Protein Names:Solute carrier family 6 member 5
Protein Family:Sodium- and chloride-dependent glycine transporter
UniProt Gene Name:SLC6A5  
Anti-SLC6A5 Antibody (CAB11647)Western blot analysis of extracts of various cell lines, using SLC6A5 antibody (CAB11647) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
View AllClose

0 Reviews

View AllClose

Related Products