Antibody Name:	Anti-SLC6A3 Antibody
Antibody SKU:	CAB15236
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SLC6A3 (NP_001035.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, C6

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SLC6A3 (NP_001035.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GVGQ FSDD IQQM TGQR PSLY WRLC WKLV SPCF LLFV VVVS IVTF RPPH YGAY IFPD WANA LGWV IATS SMAM VPIY AAYK FCSL PGSF REKL AYAI APEK DREL VDRG EVRQ FTLR HWLK V
Gene ID:	6531
Uniprot:	Q01959
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	68kDa
Observed MW:	90kDa

Synonyms:

SLC6A3, DAT, DAT1, PKDYS

Background:

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.

UniProt Protein Function:	DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
UniProt Protein Details:	Protein type:Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 5p15.3 Cellular Component: axon; cell soma; cell surface; cytoplasm; flotillin complex; integral to membrane; integral to plasma membrane; plasma membrane Molecular Function:dopamine binding; dopamine transmembrane transporter activity; dopamine:sodium symporter activity; drug binding; monoamine transmembrane transporter activity; protease binding; protein binding; protein complex binding; protein N-terminus binding; protein phosphatase 2A binding; receptor binding Biological Process: adenohypophysis development; aging; dopamine biosynthetic process; dopamine catabolic process; dopamine transport; dopamine uptake; lactation; locomotory behavior; monoamine transport; neurotransmitter biosynthetic process; positive regulation of multicellular organism growth; prepulse inhibition; regulation of dopamine metabolic process; response to cAMP; response to cocaine; response to drug; response to ethanol; response to iron ion; response to nicotine; sensory perception of smell; synaptic transmission; transmembrane transport Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To
NCBI Summary:	This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]
UniProt Code:	Q01959
NCBI GenInfo Identifier:	7108463
NCBI Gene ID:	6531
NCBI Accession:	AAC50179.2
UniProt Secondary Accession:	Q01959,Q14996, A2RUN4,
UniProt Related Accession:	Q01959
Molecular Weight:	68,495 Da
NCBI Full Name:	dopamine transporter
NCBI Synonym Full Names:	solute carrier family 6 (neurotransmitter transporter), member 3
NCBI Official Symbol:	SLC6A3
NCBI Official Synonym Symbols:	DAT; DAT1; PKDYS
NCBI Protein Information:	sodium-dependent dopamine transporter
UniProt Protein Name:	Sodium-dependent dopamine transporter
UniProt Synonym Protein Names:	Solute carrier family 6 member 3
Protein Family:	D-alanine aminotransferase
UniProt Gene Name:	SLC6A3
UniProt Entry Name:	SC6A3_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC6A3 antibody (CAB15236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using SLC6A3 antibody (CAB15236) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of rat brain using SLC6A3 Rabbit pAb (CAB15236) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse brain using SLC6A3 Rabbit pAb (CAB15236) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.