Signal Transduction Antibodies 1

Anti-SLC4A8 Antibody (CAB14825)

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SKU:
CAB14825
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-SLC4A8 Antibody
Antibody SKU:CAB14825
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human SLC4A8 (NP_001035049.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, SH-SY5Y, Mouse lung, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human SLC4A8 (NP_001035049.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPAA GSNE PDGV LSYQ RPDE EAVV DQGG TSTI LNIH YEKE ELEG HRTL YVGV RMPL GRQS HRHH RTHG QKHR RRGR GKGA SQGE EGLE AL
Gene ID:9498
Uniprot:Q2Y0W8
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:71kDa/77kDa/83kDa/111kDa/117kDa/120kDa/122kDa
Observed MW:123kDa
Synonyms:SLC4A8, NBC3, NDCBE
Background:The protein encoded by this gene is a membrane protein that functions to transport sodium and bicarbonate ions across the cell membrane. The encoded protein is important for pH regulation in neurons. The activity of this protein can be inhibited by 4, 4'-Di-isothiocyanatostilbene-2, 2'-disulfonic acid (DIDS). Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SLC4A8: Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3(-) exchange with a Na(+):HCO3(-) stoichiometry of 2:1. Plays a major role in pH regulation in neurons. May be involved in cell pH regulation by transporting HCO3(-) from blood to cell. Enhanced expression in severe acid stress could be important for cell survival by mediating the influx of HCO3(-) into the cells. Also mediates lithium-dependent HCO3(-) cotransport. May be regulated by osmolarity. Belongs to the anion exchanger (TC 2.A.31) family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane

Molecular Function:inorganic anion exchanger activity; sodium:bicarbonate symporter activity

Biological Process: bicarbonate transport; regulation of intracellular pH; ion transport; transmembrane transport

NCBI Summary:The protein encoded by this gene is a membrane protein that functions to transport sodium and bicarbonate ions across the cell membrane. The encoded protein is important for pH regulation in neurons. The activity of this protein can be inhibited by 4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:Q2Y0W8
NCBI GenInfo Identifier:90403614
NCBI Gene ID:9498
NCBI Accession:NP_001035049.1
UniProt Secondary Accession:Q2Y0W8,O94843, O95233, Q004B4, Q8N3U2, Q8TC60, Q9UKX8 A0MMZ1, B4DHY0, E7EML0, F5GZ31, F5H7F5,
UniProt Related Accession:Q2Y0W8
Molecular Weight:122938
NCBI Full Name:electroneutral sodium bicarbonate exchanger 1 isoform a
NCBI Synonym Full Names:solute carrier family 4, sodium bicarbonate cotransporter, member 8
NCBI Official Symbol:SLC4A8  
NCBI Official Synonym Symbols:NBC3; NDCBE  
NCBI Protein Information:electroneutral sodium bicarbonate exchanger 1
UniProt Protein Name:Electroneutral sodium bicarbonate exchanger 1
UniProt Synonym Protein Names:Electroneutral Na(+)-driven Cl-HCO3 exchanger; Solute carrier family 4 member 8; k-NBC3
UniProt Gene Name:SLC4A8  
UniProt Entry Name:S4A8_HUMAN
Anti-SLC4A8 Antibody (CAB14825)Western blot analysis of extracts of various cell lines, using SLC4A8 antibody (CAB14825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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