Name: Anti-SLC4A4 / NBC Antibody (CAB5332)
Brand: Signal Transduction Antibodies 3
SKU: CAB5332
Availability: OutOfStock

Antibody Name:	Anti-SLC4A4 / NBC Antibody
Antibody SKU:	CAB5332
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SLC4A4 / NBC (NP_003750.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LNCaP, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SLC4A4 / NBC (NP_003750.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSTE NVEG KPSN LGER GRAR SSTF LRVV QPMF NHSI FTSA VSPA AERI RFIL GEED DSPA PPQL FTEL DELL AVDG QEME WKET ARWI KFEE KVEQ GGER WSKP HVAT LSLH SLFE LRTC MEKG SIML DREA SSLP QLVE MIVD HQIE TGLL KPEL KDKV TYTL LRKH RHQT KKSN LRSL ADIG KTVS SASR MFTN PDNG SPAM THRN LTSS SLND ISDK PEKD QLKN KFMK KLPR DAEA
Gene ID:	8671
Uniprot:	Q9Y6R1
Cellular Location:	Basolateral cell membrane, Multi-pass membrane protein
Calculated MW:	72kDa/112kDa/116kDa/121kDa/123kDa
Observed MW:	135KDa

Synonyms:	SLC4A4, HNBC1, KNBC, NBC1, NBC2, NBCe1-A, SLC4A5, hhNMC, kNBC1, pNBC
Background:	This gene encodes a sodium bicarbonate cotransporter (NBC) involved in the regulation of bicarbonate secretion and absorption and intracellular pH. Mutations in this gene are associated with proximal renal tubular acidosis. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SLC4A4: Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA); also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy. Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17). Belongs to the anion exchanger (TC 2.A.31) family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 4q21 Cellular Component: integral to plasma membrane; basolateral plasma membrane; plasma membrane Molecular Function:protein binding; inorganic anion exchanger activity; sodium:bicarbonate symporter activity Biological Process: transport; bicarbonate transport; regulation of intracellular pH; ion transport; transmembrane transport Disease: Renal Tubular Acidosis, Proximal, With Ocular Abnormalities And Mental Retardation
NCBI Summary:	This gene encodes a sodium bicarbonate cotransporter (NBC) involved in the regulation of bicarbonate secretion and absorption and intracellular pH. Mutations in this gene are associated with proximal renal tubular acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	Q9Y6R1
NCBI GenInfo Identifier:	74721543
NCBI Gene ID:	8671
NCBI Accession:	Q9Y6R1.1
UniProt Secondary Accession:	Q9Y6R1,O15153, Q8NEJ2, Q9H262, Q9NRZ1, Q9UIC0, Q9UIC1 Q9UP50, C4B714,
UniProt Related Accession:	Q9Y6R1
Molecular Weight:	1079
NCBI Full Name:	Electrogenic sodium bicarbonate cotransporter 1
NCBI Synonym Full Names:	solute carrier family 4 (sodium bicarbonate cotransporter), member 4
NCBI Official Symbol:	SLC4A4
NCBI Official Synonym Symbols:	KNBC; NBC1; NBC2; pNBC; HNBC1; hhNMC; SLC4A5; NBCe1-A
NCBI Protein Information:	electrogenic sodium bicarbonate cotransporter 1; kNBC1; Na(+)/HCO3(-) cotransporter; solute carrier family 4, sodium bicarbonate cotransporter, member 5; solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type; sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas)
UniProt Protein Name:	Electrogenic sodium bicarbonate cotransporter 1
UniProt Synonym Protein Names:	Na(+)/HCO3(-) cotransporter; Solute carrier family 4 member 4; kNBC1
Protein Family:	Electrogenic sodium bicarbonate cotransporter
UniProt Gene Name:	SLC4A4
UniProt Entry Name:	S4A4_HUMAN

	Western blot analysis of extracts of LNCaP cells, using SLC4A4 / NBC antibody (CAB5332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using SLC4A4 / NBC antibody (CAB5332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human prostate using SLC4A4 / NBC antibody (CAB5332) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using SLC4A4 / NBC antibody (CAB5332) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SLC4A4 / NBC antibody (CAB5332) at dilution of 1:100 (40x lens).