Name: Anti-SLC3A1 Antibody (CAB5500)
Brand: Signal Transduction Antibodies 3
SKU: CAB5500
Availability: OutOfStock

Antibody Name:	Anti-SLC3A1 Antibody
Antibody SKU:	CAB5500
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 416-685 of human SLC3A1 (NP_000332.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HepG2, 293T, BxPC-3, PC-12, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 416-685 of human SLC3A1 (NP_000332.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LDTV SGNS VYEV ITSW MENM PEGK WPNW MIGG PDSS RLTS RLGN QYVN VMNM LLFT LPGT PITY YGEE IGMG NIVA ANLN ESYD INTL RSKS PMQW DNSS NAGF SEAS NTWL PTNS DYHT VNVD VQKT QPRS ALKL YQDL SLLH ANEL LLNR GWFC HLRN DSHY VVYT RELD GIDR IFIV VLNF GEST LLNL HNMI SGLP AKMR IRLS TNSA DKGS KVDT SGIF LDKG EGLI FEHN TKNL LHRQ TAFR DRCF VSNR ACYS SVLN ILYT SC
Gene ID:	6519
Uniprot:	Q07837
Cellular Location:	Membrane, Single-pass type II membrane protein
Calculated MW:	35kDa/45kDa/46kDa/57kDa/63kDa/65kDa/78kDa
Observed MW:	79kDa

Synonyms:	SLC3A1, ATR1, CSNU1, D2H, NBAT, RBAT
Background:	This gene encodes a type II membrane glycoprotein which is one of the components of the renal amino acid transporter which transports neutral and basic amino acids in the renal tubule and intestinal tract. Mutations and deletions in this gene are associated with cystinuria. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

UniProt Protein Function:	SLC3A1: Involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity). May function as an activator of SLC7A9 and be involved in the high-affinity reabsorption of cystine in the kidney tubule. Defects in SLC3A1 are a cause of cystinuria type 1 (CSNU1). Cystinuria (CSNU) arises from impaired transport of cystine and dibasic amino acids through the epithelial cells of the renal tubule and gastrointestinal tract. Defects in SLC3A1 are a cause of hypotonia-cystinuria syndrome (HCS). HCS is characterized generalized hypotonia at birth, nephrolithiasis, growth hormone deficiency, minor facial dysmorphism, failure to thrive, followed by hyperphagia and rapid weight gain in late childhood.
UniProt Protein Details:	Protein type:Membrane protein, integral; Mitochondrial; Transporter Chromosomal Location of Human Ortholog: 2p16.3 Cellular Component: brush border membrane; membrane; plasma membrane Molecular Function:amino acid transmembrane transporter activity; basic amino acid transmembrane transporter activity; L-cystine transmembrane transporter activity; protein binding Biological Process: amino acid metabolic process; amino acid transport; basic amino acid transport; L-cystine transport Disease: Cystinuria
NCBI Summary:	This gene encodes a type II membrane glycoprotein which is one of the components of the renal amino acid transporter which transports neutral and basic amino acids in the renal tubule and intestinal tract. Mutations and deletions in this gene are associated with cystinuria. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q07837
NCBI GenInfo Identifier:	67472674
NCBI Gene ID:	6519
NCBI Accession:	Q07837.2
UniProt Secondary Accession:	Q07837,O00658, Q15295, Q4J6B4, Q4J6B5, Q4J6B6, Q4J6B7 Q4J6B8, Q4J6B9, Q52M92, Q52M94, A8K0S1,
UniProt Related Accession:	Q07837
Molecular Weight:	63,401 Da
NCBI Full Name:	Neutral and basic amino acid transport protein rBAT
NCBI Synonym Full Names:	solute carrier family 3 member 1
NCBI Official Symbol:	SLC3A1
NCBI Official Synonym Symbols:	D2H; ATR1; NBAT; RBAT; CSNU1
NCBI Protein Information:	neutral and basic amino acid transport protein rBAT
UniProt Protein Name:	Neutral and basic amino acid transport protein rBAT
UniProt Synonym Protein Names:	D2h; Solute carrier family 3 member 1; b(0,+)-type amino acid transport protein
Protein Family:	Neutral and basic amino acid transport protein
UniProt Gene Name:	SLC3A1
UniProt Entry Name:	SLC31_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC3A1 antibody (CAB5500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using SLC3A1 Rabbit pAb (CAB5500) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SLC3A1 Rabbit pAb (CAB5500) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using SLC3A1 antibody (CAB5500). Blue: DAPI for nuclear staining.