Description
Antibody Name: | Anti-SLC38A1 Antibody (CAB20717) |
Antibody SKU: | CAB20717 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human SLC38A1 (NP_001070952.1). |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Rat |
Positive Samples: | A-549, Rat heart |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human SLC38A1 (NP_001070952.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | MMHF KSGL ELTE LQNM TVPE DDNI SNDS NDFT EVEN GQIN SKFI SDRE SRRS LTNS HLEK KKCD EYIP GT |
Cellular Location: | |
Calculated MW: | 54kDa |
Observed MW: | 50-70KDa |
Synonyms: | ATA1, NAT2, SAT1, SNAT1 |
Background: | Amino acid transporters play essential roles in the uptake of nutrients, production of energy, chemical metabolism, detoxification, and neurotransmitter cycling. SLC38A1 is an important transporter of glutamine, an intermediate in the detoxification of ammonia and the production of urea. Glutamine serves as a precursor for the synaptic transmitter, glutamate (Gu et al., 2001 PubMed 11325958). |
Western blot analysis of extracts of various cell lines, using SLC38A1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s. |