Signal Transduction Antibodies 1

Anti-SLC29A1 Antibody (CAB13204)

(No reviews yet) Write a Review
SKU:
CAB13204
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SLC29A1 Antibody
Antibody SKU:CAB13204
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SLC29A1 (NP_001071645.1).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SLC29A1 (NP_001071645.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELSE SAFG YFIT ACAV IILT IICY LGLP RLEF YRYY QQLK LEGP GEQE TKLD LISK GEEP RAGK EESG VSVS NSQP TNES HSIK AILK NISV LAFS VCFI F
Gene ID:2030
Uniprot:Q99808
Cellular Location:Apical cell membrane, Basolateral cell membrane, Cell membrane, Multi-pass membrane protein
Calculated MW:50kDa/58kDa
Observed MW:50kDa
Synonyms:SLC29A1, ENT1
Background:This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
UniProt Protein Function:SLC29A1: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
UniProt Protein Details:

Protein type:Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: apical plasma membrane; basolateral plasma membrane; membrane; plasma membrane

Molecular Function:nucleoside transmembrane transporter activity

Biological Process: nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleoside transport

NCBI Summary:This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q99808
NCBI GenInfo Identifier:9296956
NCBI Gene ID:2030
NCBI Accession:Q99808.3
UniProt Secondary Accession:Q99808,Q5T9W9, Q9UJY2, B3KQV7, B3KQY5,
UniProt Related Accession:Q99808
Molecular Weight:58,963 Da
NCBI Full Name:Equilibrative nucleoside transporter 1
NCBI Synonym Full Names:solute carrier family 29 member 1 (Augustine blood group)
NCBI Official Symbol:SLC29A1  
NCBI Official Synonym Symbols:ENT1  
NCBI Protein Information:equilibrative nucleoside transporter 1
UniProt Protein Name:Equilibrative nucleoside transporter 1
UniProt Synonym Protein Names:Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative NBMPR-sensitive nucleoside transporter; Nucleoside transporter, es-type; Solute carrier family 29 member 1
Protein Family:Equilibrative nucleoside transporter
UniProt Gene Name:SLC29A1  
UniProt Entry Name:S29A1_HUMAN
Anti-SLC29A1 Antibody (CAB13204)Western blot analysis of extracts of U-87MG cells, using SLC29A1 antibody (CAB13204) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SLC29A1 Antibody (CAB13204)Immunohistochemistry of paraffin-embedded rat brain using SLC29A1 antibody (CAB13204) at dilution of 1:100 (40x lens).
Anti-SLC29A1 Antibody (CAB13204)Immunohistochemistry of paraffin-embedded rat kidney using SLC29A1 antibody (CAB13204) at dilution of 1:100 (40x lens).
View AllClose

0 Reviews

View AllClose

Related Products