Antibody Name:	Anti-SLC25A4/ANT1 Antibody [KO Validated] (CAB20842)
Antibody SKU:	CAB20842
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunofluorescence
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SLC25A4/ANT1 (NP_001142.2).

Application:	Western blotting, Immunofluorescence
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF7, Mouse heart, Rat heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SLC25A4/ANT1 (NP_001142.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LGGV DRHK QFWR YFAG NLAS GGAA GATS LCFV YPLD FART RLAA DVGK GAAQ REFH GLGD CIIK IFKS DGLR GLYQ GFNV SVQG IIIY RAAY FGVY DTAK G
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	33kDa
Observed MW:	33KDa

Synonyms:

SLC25A4, AAC1, ANT, ANT 1, ANT1, MTDPS12, MTDPS12A, PEO2, PEO3, PEOA2, T1

Background:

This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Mutations in this gene have been shown to result in autosomal dominant progressive external opthalmoplegia and familial hypertrophic cardiomyopathy.

	Immunofluorescence analysis of U2OS cells using [KO Validated] SLC25A4/ANT1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using [KO Validated] SLC25A4/ANT1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Western blot analysis of extracts of various cell lines, using SLC25A4/ANT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and SLC25A4/ANT1 knockout (KO) 293T cells, using SLC25A4/ANT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Western blot analysis of extracts of MCF7 cells, using SLC25A4/ANT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.