Antibody Name:	Anti-SLC25A27 Antibody
Antibody SKU:	CAB13835
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human SLC25A27 (NP_001190981.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	mouse brain, rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human SLC25A27 (NP_001190981.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSVP EEEE RLLP LTQR WPRA SKFL LSGC AATV AELA TFPL DLTK TRLQ MQGE AALA RLGD GARE SAPY RGMV RTAL GIIE EEGF LKLW QGVT PAIY RHVV YSGG RMVT YEHL REVV FGKS EDEH YPLW KSVI GGMM AGVI GQFL ANPT DLVK VQMQ MEGK RKLE GKPL RFRG VHHA FAKI LAEG GIRG LWAG WVPN IQRA ALVN MGDL TTYD TVKH YLVL NTPL EDNI MTHG LSSD LVGS HKAI Q
Gene ID:	9481
Uniprot:	O95847
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	27kDa/36kDa
Observed MW:	36kDa

Synonyms:

SLC25A27, UCP4

Background:

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SLC25A27: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. May play a role in thermoregulatory heat production and metabolism in brain. Belongs to the mitochondrial carrier family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 6p12.3 Cellular Component: mitochondrial inner membrane; mitochondrion Biological Process: generation of precursor metabolites and energy; proton transport
NCBI Summary:	Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
UniProt Code:	O95847
NCBI GenInfo Identifier:	6136097
NCBI Gene ID:	9481
NCBI Accession:	O95847.1
UniProt Secondary Accession:	O95847,Q5VTS9, Q8N518, F5GWR4,
UniProt Related Accession:	O95847
Molecular Weight:	27,210 Da
NCBI Full Name:	Mitochondrial uncoupling protein 4
NCBI Synonym Full Names:	solute carrier family 25 member 27
NCBI Official Symbol:	SLC25A27
NCBI Official Synonym Symbols:	UCP4
NCBI Protein Information:	mitochondrial uncoupling protein 4
UniProt Protein Name:	Mitochondrial uncoupling protein 4
UniProt Synonym Protein Names:	Solute carrier family 25 member 27
UniProt Gene Name:	SLC25A27

	Western blot analysis of extracts of various cell lines, using SLC25A27 antibody (CAB13835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U-2 OS cells using UCP4 antibody (CAB13835) at dilution of 1:100. Blue: DAPI for nuclear staining.