Signal Transduction Antibodies 1
Anti-SLC25A24 Antibody (CAB12138)
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- SKU:
- CAB12138
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-SLC25A24 Antibody |
| Antibody SKU: | CAB12138 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SLC25A24 (NP_037518.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BxPC-3, U-87MG, HT-29, 293T, NIH/3T3, Mouse spleen, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SLC25A24 (NP_037518.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLRW LRDF VLPT AACQ DAEQ PTRY ETLF QALD RNGD GVVD IGEL QEGL RNLG IPLG QDAE EKIF TTGD VNKD GKLD FEEF MKYL KDHE KKMK LAFK SLDK NNDG KIEA SEIV QSLQ TLGL TISE QQAE LILQ SIDV DGTM TVDW NEWR DYFL FNPV TDIE EIIR FWKH STGI DIGD SLTI PDEF TEDE KKSG QWWR QLLA |
| Gene ID: | 29957 |
| Uniprot: | Q6NUK1 |
| Cellular Location: | Mitochondrion inner membrane, Multi-pass membrane protein |
| Calculated MW: | 51kDa/53kDa |
| Observed MW: | 50kDa |
| Synonyms: | SLC25A24, APC1, SCAMC-1 |
| Background: | This gene encodes a carrier protein that transports ATP-Mg exchanging it for phosphate. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SLC25A24: Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria. Belongs to the mitochondrial carrier family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transporter; Transporter, SLC family Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion Molecular Function:ADP transmembrane transporter activity; ATP transmembrane transporter activity; calcium ion binding Biological Process: ATP transport; mitochondrial transport |
| NCBI Summary: | This gene encodes a carrier protein that transports ATP-Mg exchanging it for phosphate. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q6NUK1 |
| NCBI GenInfo Identifier: | 167016554 |
| NCBI Gene ID: | 29957 |
| NCBI Accession: | Q6NUK1.2 |
| UniProt Secondary Accession: | Q6NUK1,Q5T331, Q5T485, Q6PJJ9, Q705K4, Q9P129, B7ZAI9 |
| UniProt Related Accession: | Q6NUK1 |
| Molecular Weight: | 53kDa |
| NCBI Full Name: | Calcium-binding mitochondrial carrier protein SCaMC-1 |
| NCBI Synonym Full Names: | solute carrier family 25 member 24 |
| NCBI Official Symbol: | SLC25A24 |
| NCBI Official Synonym Symbols: | APC1; SCAMC-1 |
| NCBI Protein Information: | calcium-binding mitochondrial carrier protein SCaMC-1 |
| UniProt Protein Name: | Calcium-binding mitochondrial carrier protein SCaMC-1 |
| UniProt Synonym Protein Names: | Mitochondrial ATP-Mg/Pi carrier protein 1; Mitochondrial Ca(2+)-dependent solute carrier protein 1; Small calcium-binding mitochondrial carrier protein 1; Solute carrier family 25 member 24 |
| Protein Family: | Calcium-binding mitochondrial carrier protein |
| UniProt Gene Name: | SLC25A24 |
 | Western blot analysis of extracts of various cell lines, using SLC25A24 antibody (CAB12138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using SLC25A24 antibody (CAB12138) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using SLC25A24 antibody (CAB12138) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using SLC25A24 antibody (CAB12138) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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