Name: Anti-SLC22A12 Antibody (CAB5118)
Brand: Signal Transduction Antibodies 2
SKU: CAB5118
Availability: OutOfStock

Antibody Name:	Anti-SLC22A12 Antibody
Antibody SKU:	CAB5118
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-150 of human SLC22A12 (NP_653186.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, HepG2, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-150 of human SLC22A12 (NP_653186.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WLCT QSML ENFS AAVP SHRC WAPL LDNS TAQA SILG SLSP EALL AISI PPGP NQRP HQCR RFRQ PQWQ LLDP NATA TSWS EADT EPCV DGWV YDRS IFTS TIVA KWNL VCDS HALK PMAQ S
Gene ID:	116085
Uniprot:	Q96S37
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	35kDa/47kDa/56kDa/59kDa
Observed MW:	75kDa

Synonyms:

SLC22A12, OAT4L, RST, URAT1

Background:

The protein encoded by this gene is a member of the organic anion transporter (OAT) family, and it acts as a urate transporter to regulate urate levels in blood. This protein is an integral membrane protein primarily found in epithelial cells of the proximal tubule of the kidney. An elevated level of serum urate, hyperuricemia, is associated with increased incidences of gout, and mutations in this gene cause renal hypouricemia type 1. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SLC22A12: Required for efficient urate re-absorption in the kidney. Regulates blood urate levels. Mediates saturable urate uptake by facilitating the exchange of urate against organic anions. Defects in SLC22A12 are the cause of hypouricemia renal type 1 (RHUC1). A disorder characterized by impaired uric acid reabsorption at the apical membrane of proximal renal tubule cells, and high urinary urate excretion. Patients often appear asymptomatic, but may be subject to exercise-induced acute renal failure, chronic renal dysfunction and nephrolithiasis. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: brush border membrane; apical plasma membrane; integral to membrane; plasma membrane Molecular Function:urate transmembrane transporter activity; PDZ domain binding Biological Process: response to drug; urate transport; urate metabolic process; cellular homeostasis; organic acid transport; transmembrane transport Disease: Hypouricemia, Renal, 1
NCBI Summary:	The protein encoded by this gene is a member of the organic anion transporter (OAT) family, and it acts as a urate transporter to regulate urate levels in blood. This protein is an integral membrane protein primarily found in epithelial cells of the proximal tubule of the kidney. An elevated level of serum urate, hyperuricemia, is associated with increased incidences of gout, and mutations in this gene cause renal hypouricemia type 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
UniProt Code:	Q96S37
NCBI GenInfo Identifier:	74732700
NCBI Gene ID:	116085
NCBI Accession:	Q96S37.1
UniProt Related Accession:	Q96S37
Molecular Weight:	Predicted Molecular Mass: 11.5kDaAccurate Molecular Mass: 12kDa
NCBI Full Name:	Solute carrier family 22 member 12
NCBI Synonym Full Names:	solute carrier family 22 member 12
NCBI Official Symbol:	SLC22A12
NCBI Official Synonym Symbols:	RST; OAT4L; URAT1
NCBI Protein Information:	solute carrier family 22 member 12
UniProt Protein Name:	Solute carrier family 22 member 12
UniProt Synonym Protein Names:	Organic anion transporter 4-like protein; Renal-specific transporter; RST; Urate anion exchanger 1
UniProt Gene Name:	SLC22A12
UniProt Entry Name:	S22AC_HUMAN

Western blot analysis of extracts of various cell lines, using SLC22A12 antibody (CAB5118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

SLC22A12 Antibody (PACO12252)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)