Antibody Name:	Anti-SLC22A11 Antibody
Antibody SKU:	CAB7816
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human SLC22A11 (NP_060954.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, BT-474, HepG2, 293T, Mouse kidney, Mouse liver, Rat kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human SLC22A11 (NP_060954.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FSAA IPGH RCWT HMLD NGSA VSTN MTPK ALLT ISIP PGPN QGPH QCRR FRQP QWQL LDPN ATAT SWSE ADTE PCVD GWVY DRSV FTST IVAK WDLV CSSQ GLKP LSQS IFM
Gene ID:	55867
Uniprot:	Q9NSA0
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	48kDa/59kDa
Observed MW:	75kDa

Synonyms:

SLC22A11, OAT4, hOAT4

Background:

The protein encoded by this gene is involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and is found mainly in the kidney and in the placenta, where it may act to prevent potentially harmful organic anions from reaching the fetus. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SLC22A11: Mediates saturable uptake of estrone sulfate, dehydroepiandrosterone sulfate and related compounds. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Transporter; Membrane protein, multi-pass; Cell surface; Transporter, SLC family Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: integral to plasma membrane; apical plasma membrane; plasma membrane; external side of plasma membrane Molecular Function:protein binding; inorganic anion exchanger activity; organic anion transmembrane transporter activity; sodium-independent organic anion transmembrane transporter activity Biological Process: urate metabolic process; transmembrane transport; organic anion transport
NCBI Summary:	The protein encoded by this gene is involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and is found mainly in the kidney and in the placenta, where it may act to prevent potentially harmful organic anions from reaching the fetus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
UniProt Code:	Q9NSA0
NCBI GenInfo Identifier:	808175981
NCBI Gene ID:	55867
NCBI Accession:	NP_001294914.1
UniProt Secondary Accession:	Q9NSA0,Q53GR2, Q6ZP72, Q8NBU4, A8K426,
UniProt Related Accession:	Q9NSA0
Molecular Weight:	60 kDa (MW of target protein)
NCBI Full Name:	solute carrier family 22 member 11 isoform 2
NCBI Synonym Full Names:	solute carrier family 22 (organic anion/urate transporter), member 11
NCBI Official Symbol:	SLC22A11
NCBI Official Synonym Symbols:	OAT4; hOAT4
NCBI Protein Information:	solute carrier family 22 member 11
UniProt Protein Name:	Solute carrier family 22 member 11
UniProt Synonym Protein Names:	Organic anion transporter 4
Protein Family:	Solute carrier family
UniProt Gene Name:	SLC22A11
UniProt Entry Name:	S22AB_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC22A11 antibody (CAB7816) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using SLC22A11 antibody (CAB7816). Blue: DAPI for nuclear staining.