Name: Anti-SLC22A1 Antibody (CAB1739)
Brand: Signal Transduction Antibodies 2
SKU: CAB1739
Availability: OutOfStock

Antibody Name:	Anti-SLC22A1 Antibody
Antibody SKU:	CAB1739
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human SLC22A1

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LO2, 293T, HT-29, Mouse liver

Immunogen:	Recombinant protein of human SLC22A1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6580
Uniprot:	O15245
Cellular Location:	Basolateral cell membrane, Multi-pass membrane protein
Calculated MW:	39kDa/53kDa/56kDa/61kDa
Observed MW:	57kDa

Synonyms:

SLC22A1, HOCT1, OCT1, oct1_cds

Background:

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter.

UniProt Protein Function:	SLC22A1: Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)- N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin- dependent kinase II and LCK tyrosine kinase. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 6q25.3 Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane Molecular Function:protein binding; protein homodimerization activity; acetylcholine transmembrane transporter activity; norepinephrine transmembrane transporter activity; organic cation transmembrane transporter activity; secondary active organic cation transmembrane transporter activity; dopamine transmembrane transporter activity; quaternary ammonium group transmembrane transporter activity Biological Process: dopamine transport; epinephrine transport; norepinephrine transport; organic cation transport; quaternary ammonium group transport; neurotransmitter transport; multidrug transport; establishment and/or maintenance of transmembrane electrochemical gradient; transmembrane transport; protein homooligomerization
NCBI Summary:	Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. [provided by RefSeq, Jul 2008]
UniProt Code:	O15245
NCBI GenInfo Identifier:	313104181
NCBI Gene ID:	6580
NCBI Accession:	O15245.2
UniProt Related Accession:	O15245
Molecular Weight:
NCBI Full Name:	Solute carrier family 22 member 1
NCBI Synonym Full Names:	solute carrier family 22 member 1
NCBI Official Symbol:	SLC22A1
NCBI Official Synonym Symbols:	OCT1; HOCT1; oct1_cds
NCBI Protein Information:	solute carrier family 22 member 1
UniProt Protein Name:	Solute carrier family 22 member 1
UniProt Synonym Protein Names:	Organic cation transporter 1; hOCT1
UniProt Gene Name:	SLC22A1
UniProt Entry Name:	S22A1_HUMAN

Western blot analysis of extracts of various cell lines, using SLC22A1 antibody (CAB1739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

ELISA Kits

OCT1 Transcription Factor Activity Assay

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)