Antibody Name:	Anti-SLC1A1 Antibody
Antibody SKU:	CAB2903
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 430-524 of human SLC1A1 (NP_004161.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 430-524 of human SLC1A1 (NP_004161.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AEDV TLII AVDW LLDR FRTM VNVL GDAF GTGI VEKL SKKE LEQM DVSS EVNI VNPF ALES TILD NEDS DTKK SYVN GGFA VDKS DTIS FTQT SQF
Gene ID:	6505
Uniprot:	P43005
Cellular Location:	Apical cell membrane, Cell membrane, Membrane, Multi-pass membrane protein
Calculated MW:	57kDa
Observed MW:	71kDa

Synonyms:

SLC1A1, DCBXA, EAAC1, EAAT3, SCZD18

Background:

This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.

UniProt Protein Function:	SLC1A1: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
UniProt Protein Details:	Protein type:Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 9p24 Cellular Component: membrane; integral to plasma membrane; apical plasma membrane; plasma membrane Molecular Function:glutamate binding; cysteine transmembrane transporter activity; sodium:dicarboxylate symporter activity; glutamate:sodium symporter activity; L-glutamate transmembrane transporter activity Biological Process: synaptic transmission; L-glutamate import; cysteine transport; ion transport; transmembrane transport; protein homooligomerization Disease: Schizophrenia 18
NCBI Summary:	This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010]
UniProt Code:	P43005
NCBI GenInfo Identifier:	1352332
NCBI Gene ID:	6505
NCBI Accession:	P43005.2
UniProt Secondary Accession:	P43005,O75587, Q5VZ24, Q8N199, Q9UEW2,
UniProt Related Accession:	P43005
Molecular Weight:	57,100 Da
NCBI Full Name:	Excitatory amino acid transporter 3
NCBI Synonym Full Names:	solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
NCBI Official Symbol:	SLC1A1
NCBI Official Synonym Symbols:	DCBXA; EAAC1; EAAT3; SCZD18
NCBI Protein Information:	excitatory amino acid transporter 3; excitatory amino acid carrier 1; solute carrier family 1 member 1; neuronal and epithelial glutamate transporter; sodium-dependent glutamate/aspartate transporter 3
UniProt Protein Name:	Excitatory amino acid transporter 3
UniProt Synonym Protein Names:	Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1
Protein Family:	Excitatory amino acid transporter
UniProt Gene Name:	SLC1A1
UniProt Entry Name:	EAA3_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC1A1 antibody (CAB2903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of rat brain using SLC1A1 antibody (CAB2903) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse brain using SLC1A1 antibody (CAB2903) at dilution of 1:100. Blue: DAPI for nuclear staining.