Signal Transduction Antibodies 1

Anti-SLC19A1 Antibody (CAB12819)

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SKU:
CAB12819
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-SLC19A1 Antibody
Antibody SKU:CAB12819
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 452-591 of human SLC19A1 (NP_919231.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:HeLa, U-87MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 452-591 of human SLC19A1 (NP_919231.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LDGL RHCQ RGHH PRQP PAQG LRSA AEEK AAQA LSVQ DKGL GGLQ PAQS PPLS PEDS LGAV GPAS LEQR QSDP YLAQ APAP QAAE FLSP VTTP SPCT LCSA QASG PEAA DETC PQLA VHPP GVSK LGLQ CLPS DGVQ NVNQ
Gene ID:6573
Uniprot:P41440
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:54kDa/60kDa/64kDa
Observed MW:65kDa
Synonyms:SLC19A1, CHMD, FOLT, IFC1, REFC, RFC1
Background:The membrane protein encoded by this gene is a transporter of folate and is involved in the regulation of intracellular concentrations of folate. Three transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SLC19A1: a transporter protein. Transports reduced folate, antitumor agents and folate antagonists such as methotrexate. Apparently involved in methotrexate resistance in various cancers and neural tube defects.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: apical plasma membrane; basolateral plasma membrane; plasma membrane

Molecular Function:folic acid binding; folic acid transporter activity; methotrexate transporter activity; reduced folate carrier activity

Biological Process: folic acid metabolic process; folic acid transport; methotrexate transport

NCBI Summary:The membrane protein encoded by this gene is a transporter of folate and is involved in the regulation of intracellular concentrations of folate. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Code:P41440
NCBI GenInfo Identifier:12643280
NCBI Gene ID:6573
NCBI Accession:P41440.3
UniProt Secondary Accession:P41440,O00553, O60227, Q13026, Q9BTX8, B2R7U8, B7Z8C3 E9PFY4,
UniProt Related Accession:P41440
Molecular Weight:Calculated MW: 54kDa/60kDa/64kDaObserved MW: 65kDa
NCBI Full Name:Folate transporter 1
NCBI Synonym Full Names:solute carrier family 19 member 1
NCBI Official Symbol:SLC19A1  
NCBI Official Synonym Symbols:CHMD; FOLT; IFC1; REFC; RFC1  
NCBI Protein Information:folate transporter 1
UniProt Protein Name:Folate transporter 1
UniProt Synonym Protein Names:Intestinal folate carrier 1; IFC-1; Placental folate transporter; Reduced folate carrier protein; RFC; Solute carrier family 19 member 1
UniProt Gene Name:SLC19A1  
Anti-SLC19A1 Antibody (CAB12819)Western blot analysis of extracts of various cell lines, using SLC19A1 antibody (CAB12819) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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