Name: Anti-SLC16A3 Antibody (CAB10548)
Brand: Signal Transduction Antibodies 1
SKU: CAB10548
Availability: OutOfStock

Antibody Name:	Anti-SLC16A3 Antibody
Antibody SKU:	CAB10548
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 371-465 of human SLC16A3 (NP_004198.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	HT-1080, SW620, MCF7, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 371-465 of human SLC16A3 (NP_004198.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PPSG GKLL DATH VYMY VFIL AGAE VLTS SLIL LLGN FFCI RKKP KEPQ PEVA AAEE EKLH KPPA DSGV DLRE VEHF LKAE PEKN GEVV HTPE TSV
Gene ID:	9123
Uniprot:	O15427
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	49kDa
Observed MW:	49kDa

Synonyms:	SLC16A3, MCT 3, MCT 4, MCT-3, MCT-4, MCT3, MCT4
Background:

UniProt Protein Function:	SLC16A3: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, SLC family Chromosomal Location of Human Ortholog: 17q25 Cellular Component: nuclear membrane; membrane; integral to plasma membrane; plasma membrane; actin cytoskeleton Molecular Function:monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity Biological Process: cellular metabolic process; monocarboxylic acid transport; pyruvate metabolic process; blood coagulation; leukocyte migration; transmembrane transport
NCBI Summary:	Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).[supplied by OMIM, Mar 2008]
UniProt Code:	O15427
NCBI GenInfo Identifier:	6225705
NCBI Gene ID:	9123
NCBI Accession:	O15427.1
UniProt Secondary Accession:	O15427,Q2M1P8, B3KXG8,
UniProt Related Accession:	O15427
Molecular Weight:
NCBI Full Name:	Monocarboxylate transporter 4
NCBI Synonym Full Names:	solute carrier family 16 (monocarboxylate transporter), member 3
NCBI Official Symbol:	SLC16A3
NCBI Official Synonym Symbols:	MCT3; MCT4; MCT 3; MCT 4; MCT-3; MCT-4
NCBI Protein Information:	monocarboxylate transporter 4; monocarboxylate transporter 3; solute carrier family 16 (monocarboxylic acid transporters), member 3; solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
UniProt Protein Name:	Monocarboxylate transporter 4
UniProt Synonym Protein Names:	Solute carrier family 16 member 3
Protein Family:	Monocarboxylate transporter
UniProt Gene Name:	SLC16A3
UniProt Entry Name:	MOT4_HUMAN

Western blot analysis of extracts of various cell lines, using SLC16A3 antibody (CAB10548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

SLC16A3 Antibody (PACO07057)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)