Name: Anti-SLC12A5 Antibody (CAB15486)
Brand: Signal Transduction Antibodies 2
SKU: CAB15486
Availability: OutOfStock

Antibody Name:	Anti-SLC12A5 Antibody
Antibody SKU:	CAB15486
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 945-1025 of human SLC12A5 (NP_001128243.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 945-1025 of human SLC12A5 (NP_001128243.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TKNE RERE IQSI TDES RGSI RRKN PANT RLRL NVPE ETAG DSEE KPEE EVQL IHDQ SAPS CPSS SPSP GEEP EGEG ETDP E
Gene ID:	57468
Uniprot:	Q9H2X9
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	123kDa/126kDa
Observed MW:	110-260kDa

Synonyms:

SLC12A5, EIEE34, EIG14, KCC2, hKCC2

Background:

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms.

UniProt Protein Function:	KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: cell soma; integral to plasma membrane; integral to membrane; plasma membrane; dendritic shaft Molecular Function:protein kinase binding; potassium:chloride symporter activity Biological Process: response to drug; synaptic transmission; transport; cellular ion homeostasis; multicellular organism growth; ion transport; learning; transmembrane transport; potassium ion transport; thermosensory behavior
NCBI Summary:	K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]
UniProt Code:	Q9H2X9
NCBI GenInfo Identifier:	161784306
NCBI Gene ID:	57468
NCBI Accession:	Q9H2X9.3
UniProt Related Accession:	Q9H2X9
Molecular Weight:
NCBI Full Name:	Solute carrier family 12 member 5
NCBI Synonym Full Names:	solute carrier family 12 member 5
NCBI Official Symbol:	SLC12A5
NCBI Official Synonym Symbols:	KCC2; EIG14; hKCC2; EIEE34
NCBI Protein Information:	solute carrier family 12 member 5
UniProt Protein Name:	Solute carrier family 12 member 5
UniProt Synonym Protein Names:	Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter
Protein Family:	Solute carrier family
UniProt Gene Name:	SLC12A5
UniProt Entry Name:	S12A5_HUMAN

Western blot analysis of extracts of various cell lines, using SLC12A5 antibody (CAB15486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-SLC12A5 Antibody (CAB15487)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)