Name: Anti-SLC12A1 Antibody (CAB12999)
Brand: Signal Transduction Antibodies 1
SKU: CAB12999
Availability: OutOfStock

Antibody Name:	Anti-SLC12A1 Antibody
Antibody SKU:	CAB12999
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_000329.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Rat kidney

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_000329.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGDE AQKR LRIS FRPG NQEC YDNF LQSG ETAK TDAS FHAY DSHT NTYY LQTF GHNT MDAV PKIE YYRN TGSI SGPK VNRP SLLE IHEQ LAKN VAVT PSSA D
Gene ID:	6557
Uniprot:	Q13621
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	121kDa
Observed MW:	150kDa

Synonyms:

SLC12A1, BSC1, NKCC2

Background:

This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.

UniProt Protein Function:	NKCC2: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Kidney specific. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 15q15-q21.1 Cellular Component: membrane; apical plasma membrane; plasma membrane; integral to membrane Molecular Function:sodium:potassium:chloride symporter activity Biological Process: transport; sodium ion transport; ion transport; chloride transport; excretion; kidney development; transmembrane transport; potassium ion transport; chemical homeostasis Disease: Bartter Syndrome, Antenatal, Type 1
NCBI Summary:	This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]
UniProt Code:	Q13621
NCBI GenInfo Identifier:	212276464
NCBI Gene ID:	6557
NCBI Accession:	Q13621.2
UniProt Related Accession:	Q13621
Molecular Weight:	Observed: 135 kDaPredicted: 122 kDa
NCBI Full Name:	Solute carrier family 12 member 1
NCBI Synonym Full Names:	solute carrier family 12 member 1
NCBI Official Symbol:	SLC12A1
NCBI Official Synonym Symbols:	BSC1; NKCC2
NCBI Protein Information:	solute carrier family 12 member 1
UniProt Protein Name:	Solute carrier family 12 member 1
UniProt Synonym Protein Names:	Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter
UniProt Gene Name:	SLC12A1
UniProt Entry Name:	S12A1_HUMAN

Western blot analysis of extracts of various cell lines, using SLC12A1 antibody (CAB12999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human SLC12A1 / Solute carrier family 12 member 1 ELISA Kit

Human Solute carrier family 12 member 1 (SLC12A1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)